MEI4 Polyclonal antibody

MEI4 Polyclonal Antibody for WB, IHC, IF-P, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Mouse, human

Applications

WB, IHC, IF-P, ELISA

Conjugate

Unconjugated

Cat no : 29892-1-AP

Synonyms

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Validation Data Gallery

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  WB analysis using 29892-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 29892-1-AP (MEI4 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse testis using 29892-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 29892-1-AP (MEI4 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse testis using 29892-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 29892-1-AP (MEI4 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of mouse testis using 29892-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse testis tissue using MEI4 antibody (29892-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: mouse heart tissue, mouse kidney tissue, mouse testis tissue
• Positive IHC detected in: mouse testis tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF-P detected in: mouse testis tissue

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:8000
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)-P: IF-P : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

29892-1-AP targets MEI4 in WB, IHC, IF-P, ELISA applications and shows reactivity with Mouse, human samples.

• Tested Reactivity: Mouse, human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: MEI4 fusion protein Ag31859
• Full Name: Meiosis-specific protein MEI4
• Calculated Molecular Weight: 44 kDa
• Observed Molecular Weight: 45 kDa
• GenBank Accession Number: NM_001322247.1
• Gene Symbol: MEI4
• Gene ID (NCBI): 101928601
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.