MEI1 Polyclonal antibody

MEI1 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 13456-1-AP

Synonyms

MEI1, Meiosis defective protein 1, meiosis inhibitor 1, Meiosis inhibitor protein 1

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Validation Data Gallery

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  WB analysis of mouse brain using 13456-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 13456-1-AP (MEI1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human cervical cancer using 13456-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 13456-1-AP (MEI1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human cervical cancer using 13456-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 13456-1-AP (MEI1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat testis using 13456-1-AP

  Immunohistochemical analysis of paraffin-embedded rat testis tissue slide using 13456-1-AP (MEI1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat testis using 13456-1-AP

  Immunohistochemical analysis of paraffin-embedded rat testis tissue slide using 13456-1-AP (MEI1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human urothelial carcinoma using 13456-1-AP

  Immunohistochemical analysis of paraffin-embedded human urothelial carcinoma tissue slide using 13456-1-AP (MEI1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human urothelial carcinoma using 13456-1-AP

  Immunohistochemical analysis of paraffin-embedded human urothelial carcinoma tissue slide using 13456-1-AP (MEI1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: mouse brain tissue
• Positive IHC detected in: human cervical cancer tissue, human urothelial carcinoma tissue, rat testis tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

13456-1-AP targets MEI1 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: MEI1 fusion protein Ag4253
• Full Name: meiosis inhibitor 1
• Calculated Molecular Weight: 894 aa, 99 kDa
• Observed Molecular Weight: 63 kDa
• GenBank Accession Number: BC032248
• Gene Symbol: MEI1
• Gene ID (NCBI): 150365
• RRID: AB_2877951
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

MEI1 may play a role in meiosis during spermatogenesis. It is required for normal meiotic chromosome synapsis. MEI1 may be involved in the formation of meiotic double-strand breaks (DSBs) in spermatocytes. This antibody recognizes isoform 1, 2, 3, 5, and 7 of MEI1 gene with MW 141 kDa, 67-72 kDa,49-54 kDa and 26 kDa .