MEF2D Polyclonal antibody

• Featured Product
• KD/KO Validated

MEF2D Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (1)

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Publications

11

Reviews (1)

Cat no : 14353-1-AP

Synonyms

Myocyte-specific enhancer factor 2D

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Validation Data Gallery

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  WB analysis of C2C12 using 14353-1-AP

  C2C12 cell were subjected to SDS PAGE followed by western blot with 14353-1-AP (MEF2D Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 14353-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 14353-1-AP (MEF2D Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  IHC staining of mouse brain using 14353-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 14353-1-AP (MEF2D antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 14353-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 14353-1-AP (MEF2D antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HeLa using 14353-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using MEF2D antibody (14353-1-AP) at dilution of 1:600 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-Phalloidin (red).

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Tested Applications

• Positive WB detected in: C2C12 cell, HeLa cells
• Positive IHC detected in: mouse brain tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:200-1:800
• Immunofluorescence (IF)/ICC: IF/ICC : 1:300-1:1200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 11 publications below
• IHC: See 2 publications below
• IF: See 2 publications below

Product Information

14353-1-AP targets MEF2D in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: MEF2D fusion protein Ag5700
• Full Name: myocyte enhancer factor 2D
• Calculated Molecular Weight: 56 kDa
• Observed Molecular Weight: 55-60 kDa
• GenBank Accession Number: BC040949
• Gene Symbol: MEF2D
• Gene ID (NCBI): 4209
• RRID: AB_2878046
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

MEF2D, also named as Myocyte-specific enhancer factor 2D, is a 521 amino acid protein, which belongs to the MEF2 family. MEF2D is expressed in t in myotubes and also in undifferentiated myoblasts. MEF2D as a transcriptional activator which binds specifically to the MEF2 element, found in numerous muscle-specific and stress-induced genes. MEF2D mediates cellular functions not only in skeletal and cardiac muscle development, but also in neuronal differentiation and survival. The calculated molecular weight of MEF2D is 55 kDa, but Phosphorylated MEF2D is 60 kDa.

Publications

Species	Application	Title
mouse	WB,IF	Nat Commun; Alternatively spliced exon regulates context-dependent MEF2D higher-order assembly during myogenesis; Authors - Mónika Gönczi
human	WB	Autophagy; MEF2D-NR4A1-FAM134B2-mediated reticulophagy contributes to amino acid homeostasis; Authors - Yuji Shiozaki
rat	WB	Ecotoxicol Environ Saf; GPX4 degradation via chaperone-mediated autophagy contributes to antimony-triggered neuronal ferroptosis.; Authors - Shali Yu
human	WB,IF	J Cell Mol Med; DYRK1A phosphorylates MEF2D and decreases its transcriptional activity.; Authors - Pin Wang
mouse	WB,IHC	J Transl Med; Extracellular vesicle miR-32 derived from macrophage promotes arterial calcification in mice with type 2 diabetes via inhibiting VSMC autophagy.; Authors - Jingsong Cao
mouse	WB	J Cell Physiol; PMID: 30488495; Authors - Jiali Nie

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Chao (Verified Customer) (03-12-2020)

Weak but predicted size band in adult mouse heart.

• Applications: Western Blot,
• Cell Tissue Type: Mouse Heart