MEF2C-Specific Polyclonal antibody

MEF2C-Specific Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 18293-1-AP

Synonyms

MEF2C, MEF2C-Specific, myocyte enhancer factor 2C

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Validation Data Gallery

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  WB analysis of SH-SY5Y using 18293-1-AP

  SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 18293-1-AP (MEF2C-Specific antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human testis using 18293-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 18293-1-AP (MEF2C-Specific antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human testis using 18293-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 18293-1-AP (MEF2C-Specific antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human brain using 18293-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 18293-1-AP (MEF2C-Specific antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human brain using 18293-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 18293-1-AP (MEF2C-Specific antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human spleen using 18293-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 18293-1-AP (MEF2C-Specific antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human spleen using 18293-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 18293-1-AP (MEF2C-Specific antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human lung using 18293-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung using 18293-1-AP (MEF2C-Specific antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human lung using 18293-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung using 18293-1-AP (MEF2C-Specific antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human kidney using 18293-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 18293-1-AP (MEF2C-Specific antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human kidney using 18293-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 18293-1-AP (MEF2C-Specific antibody) at dilution of 1:50 (under 40x lens).

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Tested Applications

• Positive WB detected in: SH-SY5Y cells
• Positive IHC detected in: human testis tissue, human brain tissue, human kidney tissue, human lung tissue, human spleen tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

18293-1-AP targets MEF2C-Specific in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: myocyte enhancer factor 2C
• Calculated Molecular Weight: 51 kDa
• Observed Molecular Weight: 45-70 kDa
• GenBank Accession Number: NM_002397
• Gene Symbol: MEF2C
• Gene ID (NCBI): 4208
• RRID: AB_2142858
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

MEF2C belongs to the MEF2 family. It is a transcription activator which binds specifically to the MEF2 element present in the regulatory regions of many muscle-specific genes. MEF2C controls cardiac morphogenesis and myogenesis, and is also involved in vascular development. It plays an essential role in hippocampal-dependent learning and memory by suppressing the number of excitatory synapses and thus regulating basal and evoked synaptic transmission. It is crucial for normal neuronal development, distribution, and electrical activity in the neocortex and is necessary for proper development of megakaryocytes and platelets and for bone marrow B lymphopoiesis. This protein is required for B-cell survival and proliferation in response to BCR stimulation, efficient IgG1 antibody responses to T-cell-dependent antigens and for normal induction of germinal center B cells. It may also be involved in neurogenesis and in the development of cortical architecture. The immunogen used is peptide from the specific sequecne of MEF2C transcript variant 1 match to transcript variant 5. The antibody can recognize the transcript variant 1, transcript variant 2 and transcript variant 3 of MEF2C.