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MED9 Polyclonal antibody

MED9 Polyclonal Antibody for WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB,ELISA

Conjugate

Unconjugated

Cat no : 27452-1-AP

Synonyms

MED25, MED9, mediator complex subunit 9

Validation Data Gallery

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  • Western Blot (WB) analysis of HEK-293T cells using MED9 Polyclonal antibody (27452-1-AP)

    WB analysis of HEK-293T using 27452-1-AP

    HEK-293T cells were subjected to SDS PAGE followed by western blot with 27452-1-AP (MED9 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

Tested Applications

Positive WB detected inHEK-293T cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

27452-1-AP targets MED9 in WB,ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen MED9 fusion protein Ag26158
Full Name mediator complex subunit 9
Calculated Molecular Weight 146 aa, 16 kDa
Observed Molecular Weight 16 kDa
GenBank Accession NumberBC000647
Gene Symbol MED9
Gene ID (NCBI) 55090
RRIDAB_2880873
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

MED9, also named as MED25, is a 146 amino acid protein, which belongs to the Mediator complex subunit 9 family. MED9 is a component of the Mediator complex and a coactivator that is involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. MED9 as a mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery.

Protocols

Product Specific Protocols
WB protocol for MED9 antibody 27452-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

FH

siting (Verified Customer) (06-24-2021)

band near to 15kb-10kb

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:500
  • Cell Tissue Type: mice heart