MED8 Polyclonal antibody

MED8 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Publications

1

Reviews (2)

Cat no : 12182-1-AP

Synonyms

ARC32, MED8, mediator complex subunit 8

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Validation Data Gallery

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  WB analysis of HepG2 using 12182-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 12182-1-AP (MED8 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse liver using 12182-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 12182-1-AP (MED8 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of SGC-7901 using 12182-1-AP

  SGC-7901 cells were subjected to SDS PAGE followed by western blot with 12182-1-AP (MED8 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human brain using 12182-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 12182-1-AP (MED8 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human brain using 12182-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 12182-1-AP (MED8 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: HepG2 cells, mouse liver tissue, SGC-7901 cells
• Positive IHC detected in: human brain tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below

Product Information

12182-1-AP targets MED8 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: MED8 fusion protein Ag2827
• Full Name: mediator complex subunit 8
• Calculated Molecular Weight: 212 aa, 23 kDa
• Observed Molecular Weight: 29 kDa
• GenBank Accession Number: BC010543
• Gene Symbol: MED8
• Gene ID (NCBI): 112950
• RRID: AB_2877834
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

MED8, a component of the Mediator complex, is a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. It plays a role as a target recruitment subunit in E3 ubiquitin-protein ligase complexes and thus in ubiquitination and subsequent proteasomal degradation of target proteins. MED8 has three isoforms with MW 19 kDa, 29 kDa and 33 kDa. And the ubiquitination form will be 38-43 kDa.

Publications

Species	Application	Title
mouse	WB	Cell Rep; The Mediator subunit MED20 organizes the early adipogenic complex to promote development of adipose tissues and diet-induced obesity.; Authors - Wen-Shuai Tang

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

siting (Verified Customer) (06-24-2021)

clear band

• Applications: Western Blot
• Primary Antibody Dilution: 1:500
• Cell Tissue Type: mice heart

MED8 Antibody Western Blot validation (1:500 dilution) in mice heart (Cat no:12182-1-AP)

FH

SITING (Verified Customer) (05-25-2021)

GOOD, SOMETIME WILL show some unspecific bands

• Applications: Western Blot
• Primary Antibody Dilution: 1:500
• Cell Tissue Type: EMBRYO HEART

MED8 Antibody Western Blot validation (1:500 dilution) in EMBRYO HEART (Cat no:12182-1-AP)