MED6 Monoclonal antibody, PBS Only

MED6 Monoclonal Antibody for WB, Indirect ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

Human, mouse, rat

Applications

WB, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

3E4G10

Cat no : 68352-1-PBS

Synonyms

ARC33, hMed6, MED6, mediator complex subunit 6, NY REN 28

---

Validation Data Gallery

• 

  WB analysis using 68352-1-Ig (same clone as 68352-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 68352-1-Ig (MED6 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Beta Actin Monoclonal antibody (HRP-66009) as loading control. This data was developed using the same antibody clone with 68352-1-PBS in a different storage buffer formulation.

---

Product Information

68352-1-PBS targets MED6 in WB, Indirect ELISA applications and shows reactivity with Human, mouse, rat samples.

• Tested Reactivity: Human, mouse, rat
• Host / Isotype: Mouse / IgG2b
• Class: Monoclonal
• Type: Antibody
• Immunogen: MED6 fusion protein Ag7645
• Full Name: mediator complex subunit 6
• Calculated Molecular Weight: 28 kDa
• Observed Molecular Weight: 33 kDa
• GenBank Accession Number: BC004106
• Gene Symbol: MED6
• Gene ID (NCBI): 10001
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

MED6 is a part of mediator complex. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors.