MED4 Polyclonal antibody

MED4 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 15951-1-AP

Synonyms

ARC36, DRIP36, HSPC126, MED4, mediator complex subunit 4, RP11 90M2.2, TRAP36, VDRIP

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Validation Data Gallery

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  WB analysis of Jurkat using 15951-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 15951-1-AP (MED4 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 15951-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 15951-1-AP (MED4 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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Tested Applications

• Positive WB detected in: Jurkat cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

15951-1-AP targets MED4 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: MED4 fusion protein Ag8629
• Full Name: mediator complex subunit 4
• Calculated Molecular Weight: 270 aa, 30 kDa
• Observed Molecular Weight: 30-35 kDa
• GenBank Accession Number: BC005189
• Gene Symbol: MED4
• Gene ID (NCBI): 29079
• RRID: AB_10640427
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.