MED30 Polyclonal antibody

• Featured Product
• KD/KO Validated

MED30 Polyclonal Antibody for WB, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IP, IHC, ELISA

Conjugate

Unconjugated

Publications

4

Reviews (1)

Cat no : 16787-1-AP

Synonyms

MED30, mediator complex subunit 30, THRAP6, TRAP25

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Validation Data Gallery

• 

  WB analysis of human heart using 16787-1-AP

  human heart tissue were subjected to SDS PAGE followed by western blot with 16787-1-AP (MED30 antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 16787-1-AP

  WB result of MED30 antibody (16787-1-AP; 1:600; incubated at room temperature for 1.5 hours) with sh-Control and sh-MED30 transfected HEK-293 cells.

• 

  IP experiment of mouse heart using 16787-1-AP

  IP result of anti-MED30 (IP:16787-1-AP, 4ug; Detection:16787-1-AP 1:500) with mouse heart tissue lysate 4000ug.

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Tested Applications

• Positive WB detected in: human heart tissue, HEK-293 cells
• Positive IP detected in: mouse heart tissue

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 2 publications below
• WB: See 3 publications below
• IHC: See 4 publications below

Product Information

16787-1-AP targets MED30 in WB, IP, IHC, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: MED30 fusion protein Ag10221
• Full Name: mediator complex subunit 30
• Calculated Molecular Weight: 178 aa, 20 kDa
• Observed Molecular Weight: 20-25 kDa
• GenBank Accession Number: BC008226
• Gene Symbol: MED30
• Gene ID (NCBI): 90390
• RRID: AB_2142439
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

MED30, also named as THRAP6 and TRAP25, is a component of the Mediator complex. MED30 has two isoforms with MW 16-20 kDa ad 20-25 kDa.

Publications

Species	Application	Title
human	IHC	J Autoimmun; Immune complexome analysis of a rich variety of serum immune complexes identifies disease-characteristic immune complex antigens in systemic sclerosis; Authors - Yuki Jimbayashi Kutsuna
human	WB, IHC	PLoS One; MED30 Regulates the Proliferation and Motility of Gastric Cancer Cells.; Authors - Yong Joo Lee
human	WB,IHC	Anticancer Res; The Contrasting Role of the Mediator Subunit MED30 in the Progression of Bladder Cancer.; Authors - Isabella Syring ; KD Validated
human	WB,IHC	Ann Diagn Pathol; The knockdown of the mediator complex subunit MED30 suppresses the proliferation and migration of renal cell carcinoma cells.; Authors - Isabella Syring ; KD Validated

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Tongbin (Verified Customer) (08-25-2020)

This antibody detects a MED30 band at around 22kD.

• Applications: Western Blot
• Cell Tissue Type: Heart