MED26 Polyclonal antibody

• Featured Product
• KD/KO Validated

MED26 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, ELISA

Conjugate

Unconjugated

Publications

3

Reviews (2)

Cat no : 21043-1-AP

Synonyms

ARC70, CRSP complex subunit 7, CRSP7, CRSP70, MED26, mediator complex subunit 26

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Validation Data Gallery

• 

  WB analysis using 21043-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 21043-1-AP (MED26 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse ovary using 21043-1-AP

  mouse ovary tissue were subjected to SDS PAGE followed by western blot with 21043-1-AP (MED26 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 21043-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 21043-1-AP (MED26 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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Tested Applications

• Positive WB detected in: mouse brain tissue, mouse ovary tissue, HEK-293 cells, rat brain tissue

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 3 publications below

Product Information

21043-1-AP targets MED26 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: MED26 fusion protein Ag15381
• Full Name: mediator complex subunit 26
• Calculated Molecular Weight: 600 aa, 65 kDa
• Observed Molecular Weight: 66-70 kDa
• GenBank Accession Number: BC110430
• Gene Symbol: MED26
• Gene ID (NCBI): 9441
• RRID: AB_10733342
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
human	WB	Nucleic Acids Res; Multiple P-TEFbs cooperatively regulate the release of promoter-proximally paused RNA polymerase II.; Authors - Xiaodong Lu ; KD Validated
	WB	bioRxiv; Comparative analysis of retroviral Gag-host cell interactions: focus on the nuclear interactome; Authors - Gregory S Lambert
	WB	Retrovirology; Comparative analysis of retroviral Gag-host cell interactions: focus on the nuclear interactome; Authors - Gregory S Lambert

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

siting (Verified Customer) (06-24-2021)

clear band

• Applications: Western Blot
• Primary Antibody Dilution: 1:500
• Cell Tissue Type: mice heart

MED26 Antibody Western Blot validation (1:500 dilution) in mice heart (Cat no:21043-1-AP)

FH

SITING (Verified Customer) (05-25-2021)

not very good in our system

• Applications: Western Blot
• Primary Antibody Dilution: 1:500
• Cell Tissue Type: EMBRYO HEART

MED26 Antibody Western Blot validation (1:500 dilution) in EMBRYO HEART (Cat no:21043-1-AP)