MED13-Specific Polyclonal antibody

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  WB analysis using 20029-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 20029-1-AP (MED13-Specific antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

MED13-Specific Polyclonal Antibody for WB, ELISA

Cat No. 20029-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, ELISA

Synonyms

ARC250, DRIP250, HSPC221, KIAA0593, MED13, MED13-Specific, mediator complex subunit 13, THRAP1, TRAP240

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Tested Applications

• Positive WB detected in: A431 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

20029-1-AP targets MED13-Specific in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: mediator complex subunit 13
• Calculated Molecular Weight: 239 kDa
• Observed Molecular Weight: 240-250 kDa
• GenBank Accession Number: NM_005121
• Gene Symbol: MED13
• Gene ID (NCBI): 9969
• RRID: AB_3085595
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q9UHV7
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

MED13, also named as ARC250, KIAA0593, THRAP1 and TRAP240, belongs to the Mediator complex subunit 13 family. MED13 is a component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors. This antibody is specific to MED13. It has no cross reaction to MED13L.