MED12L Polyclonal antibody

MED12L Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 31041-1-AP

Synonyms

FLJ46288, KIAA1635, MED12L, NOPAR, TNRC11L, TRALP, TRALPUSH

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Validation Data Gallery

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  WB analysis using 31041-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 31041-1-AP (MED12L antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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Tested Applications

• Positive WB detected in: HeLa cells, HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

31041-1-AP targets MED12L in WB, ELISA applications and shows reactivity with Human samples.

• Tested Reactivity: Human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: MED12L fusion protein Ag34636
• Full Name: mediator complex subunit 12-like
• Calculated Molecular Weight: 240 kDa
• Observed Molecular Weight: 260 kDa
• GenBank Accession Number: NM_053002
• Gene Symbol: MED12L
• Gene ID (NCBI): 116931
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

MED12L, also named as KIAA1635, TNRC11L, TRALP and TRALPUSH, belongs to the Mediator complex subunit 12 family. MED12L may be a component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. MED12L is a mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. MED12L is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors.