MECR Polyclonal antibody

• Featured Product
• KD/KO Validated

MECR Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Publications

1

Cat no : 14932-1-AP

Synonyms

HsNrbf 1, FASN2B, Enoyl-[acyl-carrier-protein] reductase, mitochondrial, EC:1.3.1.104, CGI-63

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Validation Data Gallery

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  WB analysis of mouse heart using 14932-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 14932-1-AP (MECR antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human stomach using 14932-1-AP

  human stomach tissue were subjected to SDS PAGE followed by western blot with 14932-1-AP (MECR antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IHC staining of human heart using 14932-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 14932-1-AP (MECR Antibody) at dilution of 1:200 (under 10x lens).

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  IF Staining of C2C12 using 14932-1-AP

  Immunofluorescent analysis of (4% PFA) fixed C2C12 cells using MECR antibody (14932-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

• 

  IF Staining of LNCaP using 14932-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed LNCaP cells using 14932-1-AP (MECR antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: mouse heart tissue, human stomach tissue
• Positive IHC detected in: human heart tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: C2C12 cells, LNCaP cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2400
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 1 publications below

Product Information

14932-1-AP targets MECR in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, pig
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: MECR fusion protein Ag6738
• Full Name: mitochondrial trans-2-enoyl-CoA reductase
• Calculated Molecular Weight: 40 kDa
• Observed Molecular Weight: 37 kDa
• GenBank Accession Number: BC001419
• Gene Symbol: MECR
• Gene ID (NCBI): 51102
• RRID: AB_2235303
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
human,mouse,pig	WB	PLoS Biol; Alternative splicing liberates a cryptic cytoplasmic isoform of mitochondrial MECR that antagonizes influenza virus; Authors - Steven F Baker ; KO Validated