ME1 Monoclonal antibody, PBS Only

ME1 Monoclonal Antibody for WB, IF/ICC, FC (Intra), Indirect ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

human, mouse, rat, pig, rabbit

Applications

WB, IF/ICC, FC (Intra), Indirect ELISA

Conjugate

Unconjugated

CloneNo.

2G9G9

Cat no : 68117-1-PBS

Synonyms

NADP-ME, NADP-dependent malic enzyme, Malic enzyme 1, EC:1.1.1.40, 2G9G9

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Validation Data Gallery

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  WB analysis using 68117-1-Ig (same clone as 68117-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 68117-1-Ig (ME1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 68117-1-PBS in a different storage buffer formulation.

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  WB analysis using 68117-1-Ig (same clone as 68117-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 68117-1-Ig (ME1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 68117-1-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 68117-1-Ig (same clone as 68117-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using ME1 antibody (68117-1-Ig, Clone: 2G9G9 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1). This data was developed using the same antibody clone with 68117-1-PBS in a different storage buffer formulation.

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  FC experiment of HeLa using 68117-1-Ig (same clone as 68117-1-PBS)

  1x10^6 HeLa cells were intracellularly stained with 0.8 ug ME1 Monoclonal antibody (68117-1-Ig, Clone:2G9G9) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1)(red), or 0.8 ug Mouse IgG2a isotype control Mouse McAb (66360-2-Ig, Clone: 11A1B2) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 68117-1-PBS in a different storage buffer formulation.

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Product Information

68117-1-PBS targets ME1 in WB, IF/ICC, FC (Intra), Indirect ELISA applications and shows reactivity with human, mouse, rat, pig, rabbit samples.

• Tested Reactivity: human, mouse, rat, pig, rabbit
• Host / Isotype: Mouse / IgG2a
• Class: Monoclonal
• Type: Antibody
• Immunogen: ME1 fusion protein Ag9916
• Full Name: malic enzyme 1, NADP(+)-dependent, cytosolic
• Calculated Molecular Weight: 572 aa, 64 kDa
• Observed Molecular Weight: 55-64 kDa
• GenBank Accession Number: BC025246
• Gene Symbol: ME1
• Gene ID (NCBI): 4199
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

ME1(NADP-dependent malic enzyme) belongs to the malic enzymes family. This malic enzyme catalyzes the reversible oxidative decarboxylation of malate and is a link between the glycolytic pathway and the citric acid cycle. The reaction is L-malate plus NADP(+) to form pyruvate, CO(2), and NADPH.