MDMX/MDM4 Recombinant antibody, PBS Only

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  IF Staining of U2OS using 84534-6-RR (same clone as 84534-6-PBS)

  Immunofluorescent analysis of (4% PFA) fixed U2OS cells using MDMX/MDM4 antibody (84534-6-RR, Clone: 241936A3 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 84534-6-PBS in a different storage buffer formulation.

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  IF Staining of A549 using 84534-6-RR (same clone as 84534-6-PBS)

  Immunofluorescent analysis of (4% PFA) fixed A549 cells using MDMX/MDM4 antibody (84534-6-RR, Clone: 241936A3 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 84534-6-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 84534-6-RR

  Biolayer interferometry (BLl) kinetic assays of 84534-6-RR against Human MDMX/MDM4 were performed. The affinity constant is 2.24 nM.

MDMX/MDM4 {ptg:Brand} Recombinant Antibody for IF/ICC, Indirect ELISA

Cat No. 84534-6-PBS

Clone No.241936A3

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF/ICC, Indirect ELISA

Synonyms

MDM4, MDMX, 241936A3, HDMX, Mdm2-like p53-binding protein

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Product Information

84534-6-PBS targets MDMX/MDM4 in IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: MDMX/MDM4 fusion protein Ag26678
• Full Name: Mdm4 p53 binding protein homolog (mouse)
• Calculated Molecular Weight: 490 aa, 55 kDa
• GenBank Accession Number: BC067299
• Gene Symbol: MDMX/MDM4
• Gene ID (NCBI): 4194
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• UNIPROT ID: O15151
• Storage Buffer: PBS only , pH 7.3
• Storage Conditions: Store at -80°C.

Background Information

MDM4 is also named as MDMX. It inhibits the activity of the tumor suppressor p53 by primarily cooperating with the p53 feedback regulator MDM2. MDM4 resides mostly in the cytoplasm, but can be recruited to the nucleus by MDM2.(PMID:16511572). The predicted mass of the protein is 54 kDa, while the observed mass is 70-80 kDa, a difference which stated is probably due to phosphorylation or other posttranslational modification(PMID:9226370, 28097652). It can be ubiquitinated and degraded by MDM2(PMID:16163388). It has 5 isoforms produced by alternative splicing.