MDMX/MDM4 Recombinant antibody, PBS Only

MDMX/MDM4 Uni-rAb^TM Recombinant Antibody for WB, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

241936D11

Cat no : 84534-5-PBS

Synonyms

MDMX, MDM4, p53-binding protein Mdm4, Mdm2-like p53-binding protein, HDMX

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Validation Data Gallery

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  WB analysis using 84534-5-RR (same clone as 84534-5-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 84534-5-RR (MDMX/MDM4 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84534-5-PBS in a different storage buffer formulation.

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Product Information

The immunogen of 84534-5-PBS is MDMX/MDM4 Fusion Protein expressed in E. coli.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: MDMX/MDM4 fusion protein Ag26678
• Full Name: Mdm4 p53 binding protein homolog (mouse)
• Calculated Molecular Weight: 490 aa, 55 kDa
• Observed Molecular Weight: 70-76 kDa
• GenBank Accession Number: BC067299
• Gene Symbol: MDMX/MDM4
• Gene ID (NCBI): 4194
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

MDM4 is also named as MDMX. It inhibits the activity of the tumor suppressor p53 by primarily cooperating with the p53 feedback regulator MDM2. MDM4 resides mostly in the cytoplasm, but can be recruited to the nucleus by MDM2.(PMID:16511572). The predicted mass of the protein is 54 kDa, while the observed mass is 70-80 kDa, a difference which stated is probably due to phosphorylation or other posttranslational modification(PMID:9226370, 28097652). It can be ubiquitinated and degraded by MDM2(PMID:16163388). It has 5 isoforms produced by alternative splicing.