MDMX/MDM4 Polyclonal antibody

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  WB analysis using 28747-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 28747-1-AP (MDMX antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

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  WB analysis using 28747-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 28747-1-AP (MDMX antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  IP experiment of MCF-7 using 28747-1-AP

  IP result of anti-MDMX (IP:28747-1-AP, 4ug; Detection:28747-1-AP 1:3000) with MCF-7 cells lysate 1470 ug.

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  IHC staining of human ovary tumor using 28747-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 28747-1-AP (MDM4 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human ovary tumor using 28747-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 28747-1-AP (MDMX antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 28747-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 28747-1-AP (MDM4 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

MDMX/MDM4 Polyclonal Antibody for WB, IHC, IP, ELISA

Cat No. 28747-1-AP

Publications(2)

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IP, ELISA

Synonyms

MDMX, MDM4, p53-binding protein Mdm4, Mdm2-like p53-binding protein, HDMX

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Tested Applications

• Positive WB detected in: A2780 cells, MCF-7 cells, MDA-MB-231 cells, U2OS cells
• Positive IP detected in: MCF-7 cells
• Positive IHC detected in: human ovary tumor tissue, human breast cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:12000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 2 publications below

Product Information

28747-1-AP targets MDMX/MDM4 in WB, IHC, IP, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: MDMX/MDM4 fusion protein Ag30456
• Full Name: Mdm4 p53 binding protein homolog (mouse)
• Calculated Molecular Weight: 490 aa, 55 kDa
• Observed Molecular Weight: 70-76 kDa
• GenBank Accession Number: BC067299
• Gene Symbol: MDMX/MDM4
• Gene ID (NCBI): 4194
• RRID: AB_3086083
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: O15151
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

MDM4 is also named as MDMX. It inhibits the activity of the tumor suppressor p53 by primarily cooperating with the p53 feedback regulator MDM2. MDM4 resides mostly in the cytoplasm, but can be recruited to the nucleus by MDM2.(PMID:16511572). The predicted mass of the protein is 54 kDa, while the observed mass is 70-80 kDa, a difference which stated is probably due to phosphorylation or other posttranslational modification(PMID:9226370, 28097652). It can be ubiquitinated and degraded by MDM2(PMID:16163388). It has 5 isoforms produced by alternative splicing.

Publications

Species	Application	Title
human	WB	Cancer Sci; SNRPB2 promotes triple-negative breast cancer progression by controlling alternative splicing of MDM4 pre-mRNA; Authors - Shiyi Yu
human	WB	J Med Chem; Design of Murine Double Minute 2 Proteolysis Targeting Chimera Degraders with a Built-In Tumor-Targeting Ability; Authors - Zhuqian Wang