MDM1 Recombinant antibody

MDM1 Uni-rAb^TM Recombinant Antibody for FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

230425E2

Cat no : 83141-5-RR

Synonyms

Nuclear protein MDM1, 230425E2

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Validation Data Gallery

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  FC experiment of A431 using 83141-5-RR

  1x10^6 A431 cells were intracellularly stained with 0.25 ug Mdm1 Recombinant Antibody (83141-5-RR, Clone:230425E2) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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  Affinity and Kinetic Characterization of 83141-5-RR

  Biolayer interferometry (BLl) kinetic assays of 83141-5-RR against Human MDM1 were performed. The affinity constant is 0.563 nM.

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Tested Applications

• Positive FC (Intra) detected in: A431 cells

Recommended dilution

• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 83141-5-RR is MDM1 Fusion Protein expressed in E. coli.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: MDM1 fusion protein Ag10541
• Full Name: Mdm1 nuclear protein homolog (mouse)
• Calculated Molecular Weight: 81 kDa
• GenBank Accession Number: BC028355
• Gene Symbol: MDM1
• Gene ID (NCBI): 56890
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Mdm1 is highly conserved in mammals as four sorting nexins (Snx): Snx13, 14, 19, and 25. Snx14 alleles are implicated in an autosomal-recessive neurological disease characterized by cellular vacuolization and granular plaque accumulation in late endosomes or autophagosomes. (PMID: 26283797)