MDM1 Polyclonal antibody

• Featured Product
• KD/KO Validated

MDM1 Polyclonal Antibody for WB, IHC, IF/ICC, IF-P, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IF-P, ELISA

Conjugate

Unconjugated

Publications

1

Cat no : 17575-1-AP

Synonyms

Nuclear protein MDM1

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Validation Data Gallery

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  WB analysis using 17575-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 17575-1-AP (MDM1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of Raji using 17575-1-AP

  Raji cells were subjected to SDS PAGE followed by western blot with 17575-1-AP (MDM1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human brain using 17575-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 17575-1-AP (MDM1 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human brain using 17575-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 17575-1-AP (MDM1 antibody) at dilution of 1:100 (under 40x lens).

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  IF Staining of mouse eye using 17575-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse eye tissue using MDM1 antibody (17575-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), CoraLite®594 acetylated Tubulin(Lys40) antibody (CL594-66200, Clone: 7E5H8, red).

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  IF Staining of mouse eye using 17575-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse eye tissue using MDM1 antibody (17575-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), CoraLite®594 acetylated Tubulin(Lys40) antibody (CL594-66200, Clone: 7E5H8, red).

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  IF Staining of hTERT-RPE1 using 17575-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed hTERT-RPE1 cells using MDM1 antibody (17575-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), acetylated Tubulin(Lys40) antibody (66200-1-Ig, Clone: 7E5H8, red).

• 

  IF Staining of hTERT-RPE1 using 17575-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed hTERT-RPE1 cells using MDM1 antibody (17575-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: mouse retina tissue, Raji cells, rat retina tissue
• Positive IHC detected in: human brain tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF-P detected in: mouse eye tissue
• Positive IF/ICC detected in: hTERT-RPE1 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF)-P: IF-P : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 1 publications below

Product Information

17575-1-AP targets MDM1 in WB, IHC, IF/ICC, IF-P, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: MDM1 fusion protein Ag10514
• Full Name: Mdm1 nuclear protein homolog (mouse)
• Calculated Molecular Weight: 81 kDa
• Observed Molecular Weight: 81 kDa
• GenBank Accession Number: BC028355
• Gene Symbol: MDM1
• Gene ID (NCBI): 56890
• RRID: AB_2281929
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Mdm1 was upregulated during ciliogenesis in multiciliated tracheal epithelial cells and localized to centrioles and cilia when transfected into NIH/3T3 cells. Mdm1 is a microtubule-binding protein that inhibits centrosome duplication when overexpressed. Proteomic analysis revealed that Mdm1 was also detected in the mouse photoreceptor sensory cilium complex and the outer segment (OS) of photoreceptor cells in the retina, suggesting that Mdm1 might be involved in the regulation of the functions and structure of the centrosome, as well as cilia. (PMID: 36171205)

Publications

Species	Application	Title
mouse	WB	Cell Death Dis; Mdm1 ablation results in retinal degeneration by specific intraflagellar transport defects of photoreceptor cells; Authors - Youlim Son ; KO Validated