Midkine Polyclonal antibody

• Featured Product
• KD/KO Validated

Midkine Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (1)

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Publications

12

Cat no : 11009-1-AP

Synonyms

MDK, Neurite outgrowth-promoting protein, Neurite outgrowth-promoting factor 2, Amphiregulin-associated protein

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Validation Data Gallery

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  WB analysis using 11009-1-AP

  Mouse embryo tissue were subjected to SDS PAGE followed by western blot with 11009-1-AP (Midkine antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  WB analysis using 11009-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11009-1-AP (Midkine antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of COLO 320 using 11009-1-AP

  COLO 320 cells were subjected to SDS PAGE followed by western blot with 11009-1-AP (Midkine antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse embryo using 11009-1-AP

  IP result of anti-Midkine (IP:11009-1-AP, 4ug; Detection:11009-1-AP 1:3000) with mouse embryo tissue lysate 4800 ug.

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  IHC staining of human intrahepatic cholangiocarcinoma using 11009-1-AP

  Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 11009-1-AP (Midkine antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human intrahepatic cholangiocarcinoma using 11009-1-AP

  Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 11009-1-AP (Midkine antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver cancer using 11009-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 11009-1-AP (Midkine antibody at dilution of 1:50 (under 10x lens).

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  IF Staining of HeLa using 11009-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using Midkine antibody (11009-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: mouse embryo tissue, COLO 320 cells, SH-SY5Y cells
• Positive IP detected in: mouse embryo tissue
• Positive IHC detected in: human intrahepatic cholangiocarcinoma tissue, human liver cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 3 publications below
• WB: See 8 publications below
• IHC: See 2 publications below
• IF: See 5 publications below

Product Information

11009-1-AP targets Midkine in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Midkine fusion protein Ag1465
• Full Name: midkine (neurite growth-promoting factor 2)
• Calculated Molecular Weight: 16 kDa
• Observed Molecular Weight: 16 kDa
• GenBank Accession Number: BC011704
• Gene Symbol: Midkine
• Gene ID (NCBI): 4192
• RRID: AB_2250619
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Midkine is a heparin-binding growth factor identified over 20 years ago and enhances the survival, migration and many other activities of target cells. Midkine is rich in both basic amino acids and cysteine, and is not related to most other growth factors/cytokines. It is strongly expressed during embryonic periods, especially at the midgestation stage, and plays important roles in development, especially in neurogenesis. Midkine expression in adult tissue is generally weak or undetectable, and it is induced upon injury and exerts many activities related to tissue repair. The biological activities of midkine in malignant tumors include proliferation, angiogenesis, invasion and metastasis. Various cancers express significantly higher levels of the midkine protein in early stage tumor tissues than in adjacent normal tissue.

Publications

Species	Application	Title
human	WB	Int J Mol Sci; Identification of Overexpressed Genes in Malignant Pleural Mesothelioma.; Authors - Federica Morani
human	WB,IHC,IF	Front Immunol; Roles of the m6A Modification of RNA in the Glioblastoma Microenvironment as Revealed by Single-Cell Analyses.; Authors - Feng Yuan ; KD Validated
human	WB	Front Oncol; Targeting MDK Abrogates IFN-γ-Elicited Metastasis inCancers of Various Origins.; Authors - Luyu Zheng
human	WB	Cancer Cell Int; Midkine promotes glioblastoma progression via PI3K-Akt signaling.; Authors - Beiquan Hu ; KD Validated
		Am J Respir Cell Mol Biol; Mesenchymal Glucocorticoid Receptor Regulates Development of Multiple Cell Layers of the Mouse Lung.; Authors - Bird A Daniel AD
rat	IHC	J Mol Neurosci; Electroacupuncture Alleviated Neuronal Apoptosis Following Ischemic Stroke in Rats via Midkine and ERK/JNK/p38 Signaling Pathway.; Authors - Ying Xing