MCT1 Polyclonal antibody

• Featured Product
• KD/KO Validated

MCT1 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (2)

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Publications

77

Reviews (4)

Cat no : 20139-1-AP

Synonyms

SLC16A1, Monocarboxylate transporter 1, MCT 1, MCT, HHF7

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Validation Data Gallery

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  WB analysis using 20139-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 20139-1-AP (MCT1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 20139-1-AP

  WB result of MCT1 antibody (20139-1-AP; 1:12000; incubated at room temperature for 1.5 hours) with sh-Control and sh-MCT1 transfected HeLa cells.

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  WB analysis of mouse brain using 20139-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 20139-1-AP (MCT1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse liver using 20139-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 20139-1-AP (MCT1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IP experiment of Raji using 20139-1-AP

  IP result of anti-MCT1 (IP:20139-1-AP, 4ug; Detection:20139-1-AP 1:300) with Raji cells lysate 3600 ug.

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  IHC staining of human colon cancer using 20139-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 20139-1-AP (MCT1 antibody) at dilution of 1:600 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon cancer using 20139-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 20139-1-AP (MCT1 antibody) at dilution of 1:600 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon cancer using 20139-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 20139-1-AP (MCT1 antibody) at dilution of 1:1600 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human cervical cancer using 20139-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 20139-1-AP (MCT1 antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human cervical cancer using 20139-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 20139-1-AP (MCT1 antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 20139-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 20139-1-AP (MCT1 antibody) at dilution of 1:400 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 20139-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 20139-1-AP (MCT1 antibody) at dilution of 1:400 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HepG2 using 20139-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using MCT1 antibody (20139-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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Tested Applications

• Positive WB detected in: 4T1 cells, HeLa cells, mouse brain tissue, mouse liver tissue
• Positive IP detected in: Raji cells
• Positive IHC detected in: human colon cancer tissue, human breast cancer tissue, human cervical cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:600-1:2000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 6 publications below
• WB: See 54 publications below
• IHC: See 24 publications below
• IF: See 15 publications below
• FC: See 1 publications below

Product Information

20139-1-AP targets MCT1 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, mouse, rat, bovine
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: MCT1 fusion protein Ag14098
• Full Name: solute carrier family 16, member 1 (monocarboxylic acid transporter 1)
• Calculated Molecular Weight: 500 aa, 54 kDa
• Observed Molecular Weight: 38-45 kDa
• GenBank Accession Number: BC026317
• Gene Symbol: MCT1
• Gene ID (NCBI): 6566
• RRID: AB_2878645
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

MCT1, the SLC16A1 gene product, is a trans-membrane symporter involved in lactate and pyruvate transportation. It plays an important role in lactic acid transport and H+ clearance in cancer cells. MCT overexpression had been observed in various cancers and may play an important role in tumorigenesis. Two isoforms of MCT1 exist due to the alternative splicing, with predicted MW of 54 kDa and 46 kDa, respectively. While western blot analysis detected MCT1 at an apparent molecular mass of 40-50 kDa.

Publications

Species	Application	Title
mouse	WB,IHC	Cell Metab; High dietary fructose promotes hepatocellular carcinoma progression by enhancing O-GlcNAcylation via microbiota-derived acetate; Authors - Peng Zhou
mouse	IF	Cell Metab; Acetate enables metabolic fitness and cognitive performance during sleep disruption; Authors - Qinqin He
mouse	WB	Nat Cancer; Targeting the bicarbonate transporter SLC4A4 overcomes immunosuppression and immunotherapy resistance in pancreatic cancer; Authors - Federica Cappellesso
mouse	IHC	Clin Cancer Res; A Novel Role for DNA-PK in Metabolism by Regulating Glycolysis in Castration Resistant Prostate Cancer.; Authors - Emanuela Dylgjeri
human	WB,IHC	Theranostics; ITGB2-mediated metabolic switch in CAFs promotes OSCC proliferation by oxidation of NADH in mitochondrial oxidative phosphorylation system.; Authors - Xiaoxin Zhang
rat	WB	Acta Pharmacol Sin; Pinocembrin attenuates hemorrhagic transformation after delayed t-PA treatment in thromboembolic stroke rats by regulating endogenous metabolites.; Authors - Ling-Lei Kong

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Angie (Verified Customer) (08-27-2024)

A549 lysate was subjected to western blot with MCT1 antibody used at 1:10000 dilution and incubated at room temperature for 1.5 hours. Secondary antibody Donkey-anti-rabbit (Alexa Fluor 800) used at 1:20000 dilution incubated at room temperature for 1 hour. One thick band at around 40 kDa. Image is shown in grayscale.

• Applications: Western Blot
• Primary Antibody Dilution: 1:10000
• Cell Tissue Type: A549

MCT1 Antibody Western Blot validation (1:10000 dilution) in A549 (Cat no:20139-1-AP)

FH

Xie (Verified Customer) (07-07-2023)

The expression of MCT4 was seen in my samples.

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1:250
• Cell Tissue Type: brain

MCT1 Antibody Immunofluorescence validation (1:250 dilution) in brain (Cat no:20139-1-AP)

FH

Aderonke (Verified Customer) (05-23-2023)

The expression of MCT4 was seen in three of the five cell lines used without an inhibitor. Also, an inhibtor of MCT4, was used at two different concentrations and the expression was decreased in the cells that expressed the protein.

• Applications: Western Blot
• Primary Antibody Dilution: 1:10,000
• Cell Tissue Type: Head and Neck cancer cell lines

FH

Huseyin (Verified Customer) (07-28-2022)

I used the antibody with 1:3000 dilution in TBS-T solution O/N and I received correct size bands in Ishikawa cell line.

• Applications: Western Blot
• Primary Antibody Dilution: 1:3000
• Cell Tissue Type: Ishikawa Cell Line