MCOLN1 Polyclonal antibody

MCOLN1 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Publications

2

Cat no : 15291-1-AP

Synonyms

MSTP080, MST080, MLIV, ML4, ML1

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Validation Data Gallery

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  WB analysis using 15291-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15291-1-AP (MCOLN1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse brain using 15291-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 15291-1-AP (MCOLN1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse brain using 15291-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 15291-1-AP (MCOLN1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of U2OS using 15291-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed U2OS cells using MCOLN1 antibody (15291-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: human placenta tissue, mouse kidney tissue, rat kidney tissue
• Positive IHC detected in: mouse brain tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: U2OS cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below

Product Information

15291-1-AP targets MCOLN1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: MCOLN1 fusion protein Ag7493
• Full Name: mucolipin 1
• Calculated Molecular Weight: 65 kDa
• Observed Molecular Weight: 60-65 kDa
• GenBank Accession Number: BC005149
• Gene Symbol: MCOLN1
• Gene ID (NCBI): 57192
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

MCOLN1, also named MLIV, ML4, MG-2, and Mucolipidin, belongs to the transient receptor (TC 1.A.4) family, Polycystin subfamily, and MCOLN1 sub-subfamily. It is playing a role in the endocytic pathway and in the control of membrane trafficking of proteins and lipids. MCOLN1 may play a major role in Ca2+ transport regulating lysosomal exocytosis. It is an autosomal recessive lysosomal storage disorder characterized by severe psychomotor retardation and ophthalmologic abnormalities, including corneal opacity, retinal degeneration, and strabismus.

Publications

Species	Application	Title
human		Autophagy; Periplocin suppresses the growth of colorectal cancer cells by triggering LGALS3 (galectin 3)-mediated lysophagy; Authors - Kui Wang
mouse,rat	WB	Biochim Biophys Acta Mol Basis Dis; TRPML1 ameliorates seizures-related neuronal injury by regulating autophagy and lysosomal biogenesis via Ca2+/TFEB signaling pathway; Authors - Tingting Peng