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  • KD/KO Validated

MCM3 Polyclonal antibody

MCM3 Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IP, ELISA

Conjugate

Unconjugated

Cat no : 15597-1-AP

Synonyms

HCC5, MCM3, P1 MCM3, P1.h, p102, RLF subunit beta, RLFB



Tested Applications

Positive WB detected inK-562 cells, Raji cells, HeLa cells
Positive IP detected inHeLa cells
Positive IHC detected inhuman colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:800-1:3200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

15597-1-AP targets MCM3 in WB, IHC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen MCM3 fusion protein Ag7966
Full Name minichromosome maintenance complex component 3
Calculated Molecular Weight 91 kDa
Observed Molecular Weight 102-105 kDa
GenBank Accession NumberBC001626
Gene Symbol MCM3
Gene ID (NCBI) 4172
RRIDAB_2141973
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

MCM3, also named as HCC5, P1-MCM3, p102, RLF subunit beta and RLFB, belongs to the MCM family. MCM3 is essential for the initiation of DNA replication and is involved in ensuring that DNA replication is initiated precisely once per cell cycle(PMID: 33093917). MCM3 has 2 isoforms with the molecular mass of 91 and 96 kDa. The overexpression of MCM3 is related to proliferation of tumor cells(PMID: 31186514).

Protocols

Product Specific Protocols
WB protocol for MCM3 antibody 15597-1-APDownload protocol
IHC protocol for MCM3 antibody 15597-1-APDownload protocol
IP protocol for MCM3 antibody 15597-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Nat Commun

ICAM1 initiates CTC cluster formation and trans-endothelial migration in lung metastasis of breast cancer.

Authors - Rokana Taftaf
humanWB

Biomaterials

Biocompatible PEGylated Gold nanorods function As cytokinesis inhibitors to suppress angiogenesis.

Authors - Hongyuan Song
mouse,humanWB

Aging Cell

Pyrroloquinoline quinone alleviates natural aging-related osteoporosis via a novel MCM3-Keap1-Nrf2 axis-mediated stress response and Fbn1 upregulation

Authors - Jie Li
  • KD Validated
humanWB

Elife

MCMBP promotes the assembly of the MCM2-7 hetero-hexamer to ensure robust DNA replication in human cells.

Authors - Yuichiro Saito
humanWB

Oncotarget

Lamin B2 binding to minichromosome maintenance complex component 7 promotes non-small cell lung carcinogenesis.

Authors - Yinan Ma
humanWB

Ecotoxicol Environ Saf

The influence of phenolic environmental estrogen on the transcriptome of uterine leiomyoma cells: A whole transcriptome profiling-based analysis.

Authors - Zemin Li