Filter: AllWBIHCIF/ICCAffinity

WB analysis using 84511-5-RR (same clone as 84511-5-PBS)
Various lysates were subjected to SDS PAGE followed by western blot with 84511-5-RR (MCM2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84511-5-PBS in a different storage buffer formulation.
IHC staining of mouse colon using 84511-5-RR (same clone as 84511-5-PBS)
Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 84511-5-RR (MCM2 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84511-5-PBS in a different storage buffer formulation.
IHC staining of mouse colon using 84511-5-RR (same clone as 84511-5-PBS)
Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 84511-5-RR (MCM2 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84511-5-PBS in a different storage buffer formulation.
IHC staining of human colon cancer using 84511-5-RR (same clone as 84511-5-PBS)
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 84511-5-RR (MCM2 antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84511-5-PBS in a different storage buffer formulation.
IHC staining of human colon cancer using 84511-5-RR (same clone as 84511-5-PBS)
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 84511-5-RR (MCM2 antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84511-5-PBS in a different storage buffer formulation.
IHC staining of human placenta using 84511-5-RR (same clone as 84511-5-PBS)
Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 84511-5-RR (MCM2 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84511-5-PBS in a different storage buffer formulation.
IHC staining of human placenta using 84511-5-RR (same clone as 84511-5-PBS)
Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 84511-5-RR (MCM2 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84511-5-PBS in a different storage buffer formulation.
IHC staining of mouse small intestine using 84511-5-RR (same clone as 84511-5-PBS)
Immunohistochemical analysis of paraffin-embedded mouse small intestine tissue slide using 84511-5-RR (MCM2 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84511-5-PBS in a different storage buffer formulation.
IHC staining of mouse small intestine using 84511-5-RR (same clone as 84511-5-PBS)
Immunohistochemical analysis of paraffin-embedded mouse small intestine tissue slide using 84511-5-RR (MCM2 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84511-5-PBS in a different storage buffer formulation.
IHC staining of human cervical squamous cancer using 84511-5-RR (same clone as 84511-5-PBS)
Immunohistochemical analysis of paraffin-embedded human cervical squamous cancer tissue slide using 84511-5-RR (MCM2 antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84511-5-PBS in a different storage buffer formulation.
IHC staining of human cervical squamous cancer using 84511-5-RR (same clone as 84511-5-PBS)
Immunohistochemical analysis of paraffin-embedded human cervical squamous cancer tissue slide using 84511-5-RR (MCM2 antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84511-5-PBS in a different storage buffer formulation.
IF Staining of MCF-7 using 84511-5-RR (same clone as 84511-5-PBS)
Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using MCM2 antibody (84511-5-RR, Clone: 241968F7 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 84511-5-PBS in a different storage buffer formulation.
IF Staining of HeLa using 84511-5-RR (same clone as 84511-5-PBS)
Immunofluorescent analysis of (4% PFA) fixed HeLa cells using MCM2 antibody (84511-5-RR, Clone: 241968F7 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 84511-5-PBS in a different storage buffer formulation.
Affinity and Kinetic Characterization of 84511-5-RR
Biolayer interferometry (BLl) kinetic assays of 84511-5-RR against Human MCM2 were performed. The affinity constant is 2.04 nM.

MCM2 Recombinant antibody, PBS Only

MCM2 Uni-rAb^TM Recombinant Antibody for WB, IHC, IF/ICC, Indirect ELISA

Cat No. 84511-5-PBS

Clone No.241968F7

Review Product

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

KIAA0030, EC:3.6.4.12, CDCL1, CCNL1, BM28

Formulation: PBS Only

PBS and Azide

PBS Only

Conjugate: Unconjugated

Size/Concentration:

-/ -

Freight/Packing: -

Quantity

- +

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Overview

Tested Applications
Product Information

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MSDS SDS

Product Information

84511-5-PBS targets MCM2 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Host / Isotype	Rabbit / IgG
Class	Recombinant
Type	Antibody
Immunogen	MCM2 fusion protein Ag0798 Predict reactive species
Full Name	minichromosome maintenance complex component 2
Calculated Molecular Weight	102 kDa
Observed Molecular Weight	116-125 kDa
GenBank Accession Number	BC007670
Gene Symbol	MCM2
Gene ID (NCBI)	4171
Conjugate	Unconjugated
Form	Liquid
Purification Method	Protein A purfication
UNIPROT ID	P49736
Storage Buffer	PBS Only
Storage Conditions	Store at -80°C.

Background Information

The MCM2-7 complex forms the core of the replicative helicase which acts as the molecular motor that uses ATP binding and hydrolysis to fuel the unwinding of double-stranded DNA at the replication fork in eukaryotes. This complex is the putative replicative helicase essential for 'once per cell cycle' DNA replication initiation and elongation in eukaryotic cells. MCM2, also named CDCL1 and BM28, is a human nuclear protein that plays an important role in 2 crucial steps of the cell cycle, namely, onset of DNA replication and cell division.