MCEE Polyclonal antibody

MCEE Polyclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

Publications

2

Cat no : 16499-1-AP

Synonyms

methylmalonyl CoA epimerase, MCE, GLOD2, EC:5.1.99.1, DL-methylmalonyl-CoA racemase

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Validation Data Gallery

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  WB analysis using 16499-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 16499-1-AP (MCEE antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of human heart using 16499-1-AP

  human heart tissue were subjected to SDS PAGE followed by western blot with 16499-1-AP (MCEE antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of human liver using 16499-1-AP

  human liver tissue were subjected to SDS PAGE followed by western blot with 16499-1-AP (MCEE antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human heart using 16499-1-AP

  human heart tissue were subjected to SDS PAGE followed by western blot with 16499-1-AP (MCEE antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

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  IF Staining of HepG2 using 16499-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using 16499-1-AP (MCEE antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: mouse liver tissue, human heart tissue, human liver tissue, rat liver tissue
• Positive IF/ICC detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 2 publications below

Product Information

16499-1-AP targets MCEE in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: MCEE fusion protein Ag9665
• Full Name: methylmalonyl CoA epimerase
• Calculated Molecular Weight: 176 aa, 19 kDa
• Observed Molecular Weight: 15-19 kDa
• GenBank Accession Number: BC020825
• Gene Symbol: MCEE
• Gene ID (NCBI): 84693
• RRID: AB_2143812
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
human	WB	Nat Metab; Altered propionate metabolism contributes to tumour progression and aggressiveness.; Authors - Ana P Gomes
human	WB	Biochim Biophys Acta Mol Basis Dis; ACSS3 regulates the metabolic homeostasis of epithelial cells and alleviates pulmonary fibrosis; Authors - Lan Wang