MCCC1 Polyclonal antibody

MCCC1 Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, ELISA

Conjugate

Unconjugated

Publications

2

Cat no : 14861-1-AP

Synonyms

MCC B, MCCA, MCCase subunit alpha, MCCC1

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Validation Data Gallery

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  WB analysis of mouse liver using 14861-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 14861-1-AP (MCCC1 antibody) at dilution of 1:1200 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse heart using 14861-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 14861-1-AP (MCCC1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse liver using 14861-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 14861-1-AP (MCCC1 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse liver using 14861-1-AP

  IP result of anti-MCCC1 (IP:14861-1-AP, 4ug; Detection:14861-1-AP 1:1000) with mouse liver tissue lysate 6000ug.

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  IHC staining of human liver cancer using 14861-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer using 14861-1-AP (MCCC1 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human liver cancer using 14861-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer using 14861-1-AP (MCCC1 antibody) at dilution of 1:100 (under 40x lens).

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Tested Applications

• Positive WB detected in: mouse liver tissue, mouse heart tissue
• Positive IP detected in: mouse liver tissue
• Positive IHC detected in: human liver cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2400
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 2 publications below
• IHC: See 1 publications below

Product Information

14861-1-AP targets MCCC1 in WB, IP, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: MCCC1 fusion protein Ag6663
• Full Name: methylcrotonoyl-Coenzyme A carboxylase 1 (alpha)
• Calculated Molecular Weight: 76 kDa
• Observed Molecular Weight: 70-76 kDa
• GenBank Accession Number: BC004187
• Gene Symbol: MCCC1
• Gene ID (NCBI): 56922
• RRID: AB_2143798
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

MCCC1 is the large subunit of 3-methylcrotonyl-CoA carboxylase. This enzyme functions as a heterodimer and catalyzes the carboxylation of 3-methylcrotonyl-CoA to form 3-methylglutaconyl-CoA. Mutations in this gene are associated with 3-Methylcrotonylglycinuria, an autosomal recessive disorder of leucine catabolism.

Publications

Species	Application	Title
human	WB	Biology (Basel); In Situ Peroxidase Labeling Followed by Mass-Spectrometry Reveals TIA1 Interactome.; Authors - Olga Gourdomichali
human	WB,IHC	Life Sci; Methylcrotonyl-CoA carboxylase subunit 1 (MCCA) regulates multidrug resistance in multiple myeloma; Authors - Yu Feng