MCAT Polyclonal antibody

• 

  WB analysis using 15536-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15536-1-AP (MCAT antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  IF Staining of HepG2 using 15536-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using MCAT antibody (15536-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002).

MCAT Polyclonal Antibody for WB, IF/ICC, ELISA

Cat No. 15536-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, ELISA

Synonyms

MCT, Malonyl-CoA-acyl carrier protein transacylase, mitochondrial, FASN2C, fabD, EC:2.3.1.39

---

Tested Applications

• Positive WB detected in: HEK-293 cells, HL-60 cells, MCF-7 cells
• Positive IF/ICC detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

15536-1-AP targets MCAT in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: MCAT fusion protein Ag7867
• Full Name: malonyl CoA:ACP acyltransferase (mitochondrial)
• Calculated Molecular Weight: 43 kDa
• Observed Molecular Weight: 39 kDa
• GenBank Accession Number: BC042195
• Gene Symbol: MCAT
• Gene ID (NCBI): 27349
• RRID: AB_3669211
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q8IVS2
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Malonyl-CoA-acyl carrier protein transacylase (MCAT), also known as MT, is an enzyme involved in mitochondrial fatty acid synthesis (mtFAS) and catalyzes the transfer of the malonyl moiety of malonyl-CoA to the mitochondrial acyl carrier protein (ACP) (PMID: 36881526). It has four coding exons and is transcribed into two isoforms by the alternative splicing of exon 3 (PMID: 33918393).