MBP-Tag Polyclonal antibody
MBP-Tag Polyclonal Antibody for WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
recombinant protein and More (2)
Applications
WB, IHC, IF, IP, CoIP, ELISA
Conjugate
Unconjugated
Cat no : 15089-1-AP
Synonyms
"MBP-Tag Antibodies" Comparison
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Tested Applications
| Positive WB detected in | lane1: MBP-NOL6 64kd; lane2 MBP 42kd, Recombinant protein |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:6000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 19 publications below |
| IHC | See 2 publications below |
| IF | See 4 publications below |
| IP | See 1 publications below |
| ELISA | See 1 publications below |
| CoIP | See 2 publications below |
Product Information
15089-1-AP targets MBP-Tag in WB, IHC, IF, IP, CoIP, ELISA applications and shows reactivity with recombinant protein samples.
| Tested Reactivity | recombinant protein |
| Cited Reactivity | human, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | MBP-Tag fusion protein Ag0942 |
| Full Name | MBP-Tag |
| Calculated Molecular Weight | 40 kDa |
| Gene Symbol | |
| Gene ID (NCBI) | |
| RRID | AB_1607774 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Protein tags are protein or peptide sequences located either on the C- or N- terminal of the target protein, which facilitates one or several of the following characteristics: solubility, detection, purification, localization and expression. Maltose binding protein (MBP) is the 370 amino acid product of the E.coli mal E gene. MBP is a useful affinity tag that can increase the expression level and solubility of the resulting tagged protein. The MBP tag also promotes proper folding of the attached protein. Plasmid vectors have been constructed utilizing the MBP domain that allow the synthesis of high levels of MBP-fusion proteins that can be purified in a one step procedure by affinity chromatography cross linked amylose resin. Once bound to amylose, the MBP protein can then be separated from the target protein by cleavage by coagulation Factor Xa at a specific four residue site. Alternatively, the intact fusion protein can be specifically eluted from the resin by the addition of excess free maltose. Subsequent to elution, MBP fusion protein can be visualized either by western blot analysis or immunoprecipitation using antibodies specific for the MBP-tag. This antibody recognizes MBP (Maltose binding protein) TAG in some expression systems.
Publications
| Species | Application | Title |
|---|---|---|
Blood Gamma-glutamyl carboxylase mutations differentially affect the biological function of vitamin K-dependent proteins. | ||
Blood γ-Glutamyl carboxylase mutations differentially affect the biological function of vitamin K-dependent proteins. | ||
Sci Adv The deubiquitinases UBP12 and UBP13 integrate with the E3 ubiquitin ligase XBAT35.2 to modulate VPS23A stability in ABA signaling. | ||
Proc Natl Acad Sci U S A Polyprotein strategy for stoichiometric assembly of nitrogen fixation components for synthetic biology. |
