MBOAT4 Polyclonal antibody

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  WB analysis using 32120-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 32120-1-AP (MBOAT4 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

MBOAT4 Polyclonal Antibody for WB, ELISA

Cat No. 32120-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Synonyms

OACT4, Membrane-bound O-acyltransferase domain-containing protein 4, GOAT, Ghrelin O-acyltransferase, FKSG89

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Tested Applications

• Positive WB detected in: HepG2 cells, MCF-7 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

32120-1-AP targets MBOAT4 in WB, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: MBOAT4 fusion protein Ag37237
• Full Name: membrane bound O-acyltransferase domain containing 4
• Calculated Molecular Weight: 50 kDa
• Observed Molecular Weight: 50 kDa
• GenBank Accession Number: NM_001100916
• Gene Symbol: MBOAT4
• Gene ID (NCBI): 619373
• RRID: AB_3670198
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity Purification
• UNIPROT ID: Q96T53
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

MBOAT4, also known as ghrelin O-acyltransferase (GOAT), is an endoplasmic reticulum enzyme that catalyzes the octanoylation of the peptide hormone ghrelin, which is essential for the binding of ghrelin to its receptor and the subsequent regulation of food intake, growth hormone secretion, and inhibition of insulin secretion (PMID: 34946685). MBOAT4 is considered a potential therapeutic target for treating disorders related to ghrelin signaling (PMID: 32564644).