MBIP Monoclonal antibody, PBS Only

• Featured Product
• KD/KO Validated

MBIP Monoclonal Antibody for WB, IP, IHC, Indirect ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

human, mouse

Applications

WB, IP, IHC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

2E6H11

Cat no : 66102-1-PBS

Synonyms

MBIP, MUK binding inhibitory protein

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Validation Data Gallery

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  WB analysis using 66102-1-Ig (same clone as 66102-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66102-1-Ig (MBIP antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66102-1-PBS in a different storage buffer formulation.

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  WB analysis of HEK-293 using 66102-1-Ig (same clone as 66102-1-PBS)

  WB result of MBIP antibody (66102-1-Ig; 1:2000; room temperature for 1.5 hours) with sh-Control and sh-MBIP transfected HEK-293 cells. This data was developed using the same antibody clone with 66102-1-PBS in a different storage buffer formulation.

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  WB analysis of human heart using 66102-1-Ig (same clone as 66102-1-PBS)

  human heart tissue were subjected to SDS PAGE followed by western blot with 66102-1-Ig (MBIP antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66102-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HepG2 using 66102-1-Ig (same clone as 66102-1-PBS)

  HepG2 cells were subjected to SDS PAGE followed by western blot with 66102-1-Ig (MBIP antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66102-1-PBS in a different storage buffer formulation.

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  IP experiment of HEK-293 using 66102-1-Ig (same clone as 66102-1-PBS)

  IP result of anti-MBIP (IP:66102-1-Ig, 3ug; Detection:66102-1-Ig 1:300) with HEK-293 cells lysate 1800ug. This data was developed using the same antibody clone with 66102-1-PBS in a different storage buffer formulation.

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  IHC staining of human kidney using 66102-1-Ig (same clone as 66102-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 66102-1-Ig (MBIP antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66102-1-PBS in a different storage buffer formulation.

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  IHC staining of human kidney using 66102-1-Ig (same clone as 66102-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 66102-1-Ig (MBIP antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66102-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse testis using 66102-1-Ig (same clone as 66102-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 66102-1-Ig (MBIP antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66102-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse testis using 66102-1-Ig (same clone as 66102-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 66102-1-Ig (MBIP antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66102-1-PBS in a different storage buffer formulation.

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Product Information

66102-1-PBS targets MBIP in WB, IP, IHC, Indirect ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Mouse / IgG2b
• Class: Monoclonal
• Type: Antibody
• Immunogen: MBIP fusion protein Ag18090
• Full Name: MAP3K12 binding inhibitory protein 1
• Calculated Molecular Weight: 39 kDa
• Observed Molecular Weight: 39 kDa
• GenBank Accession Number: BC005197
• Gene Symbol: MBIP
• Gene ID (NCBI): 51562
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

MBIP (MAP3K12 binding inhibitory protein 1), also known as MAPK upstream kinase (MUK)-binding inhibitory protein, is a 344 amino acid nuclear and cytoplasmic protein that assists in the activation of the JNK pathway by inhibiting ZPK activity. Ubiquitously expressed, MBIP is found at high levels in lung and heart and is one component of the ADA2A-containing complex (ATAC) in conjunction with ADA3, GCN5, CDC101, TADA2L, WDR5, YEATS2, DR1 and CRP2BP.