MBD3 Polyclonal antibody, PBS Only

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  WB analysis of Y79 using 14258-1-AP (same clone as 14258-1-PBS)

  Y79 cells were subjected to SDS PAGE followed by western blot with 14258-1-AP (MBD3 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14258-1-PBS in a different storage buffer formulation.

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  WB analysis of MCF-7 using 14258-1-AP (same clone as 14258-1-PBS)

  MCF7 cells were subjected to SDS PAGE followed by western blot with 14258-1-AP (MBD3 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14258-1-PBS in a different storage buffer formulation.

• 

  WB analysis of MCF-7 using 14258-1-AP (same clone as 14258-1-PBS)

  MCF7 cells were subjected to SDS PAGE followed by western blot with 14258-1-AP (MBD3 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14258-1-PBS in a different storage buffer formulation.

• 

  WB analysis of Y79 using 14258-1-AP (same clone as 14258-1-PBS)

  Y79 cells were subjected to SDS PAGE followed by western blot with 14258-1-AP (MBD3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14258-1-PBS in a different storage buffer formulation.

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  WB analysis of Jurkat using 14258-1-AP (same clone as 14258-1-PBS)

  Jurkat cells were subjected to SDS PAGE followed by western blot with 14258-1-AP (MBD3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14258-1-PBS in a different storage buffer formulation.

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  WB analysis of HeLa using 14258-1-AP (same clone as 14258-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 14258-1-AP (MBD3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14258-1-PBS in a different storage buffer formulation.

• 

  WB analysis of MCF-7 using 14258-1-AP (same clone as 14258-1-PBS)

  MCF7 cells were subjected to SDS PAGE followed by western blot with 14258-1-AP (MBD3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14258-1-PBS in a different storage buffer formulation.

• 

  WB analysis of COLO 320 using 14258-1-AP (same clone as 14258-1-PBS)

  COLO 320 cells were subjected to SDS PAGE followed by western blot with 14258-1-AP (MBD3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14258-1-PBS in a different storage buffer formulation.

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  WB analysis of mouse testis using 14258-1-AP (same clone as 14258-1-PBS)

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 14258-1-AP (MBD3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14258-1-PBS in a different storage buffer formulation.

• 

  WB analysis of Y79 using 14258-1-AP (same clone as 14258-1-PBS)

  Y79 cells were subjected to SDS PAGE followed by western blot with 14258-1-AP (MBD3 antibody) at dilution of 1:100 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14258-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HeLa using 14258-1-AP (same clone as 14258-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 14258-1-AP (MBD3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14258-1-PBS in a different storage buffer formulation.

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  IP experiment of mouse testis using 14258-1-AP (same clone as 14258-1-PBS)

  IP result of anti-MBD3 (IP:14258-1-AP, 4ug; Detection:14258-1-AP 1:800) with mouse testis tissue lysate 4000ug. This data was developed using the same antibody clone with 14258-1-PBS in a different storage buffer formulation.

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  IHC staining of human endometrial cancer using 14258-1-AP (same clone as 14258-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human endometrial cancer using 14258-1-AP (MBD3 antibody) at dilution of 1:100 (under 40x lens). This data was developed using the same antibody clone with 14258-1-PBS in a different storage buffer formulation.

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  IHC staining of human endometrial cancer using 14258-1-AP (same clone as 14258-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human endometrial cancer using 14258-1-AP (MBD3 antibody) at dilution of 1:100 (under 10x lens). This data was developed using the same antibody clone with 14258-1-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 14258-1-AP (same clone as 14258-1-PBS)

  Immunofluorescent analysis of HepG2 cells, using MBD3 antibody 14258-1-AP at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye). This data was developed using the same antibody clone with 14258-1-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 14258-1-AP (same clone as 14258-1-PBS)

  Immunofluorescent analysis of HepG2 cells, using MBD3 antibody 14258-1-AP at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). This data was developed using the same antibody clone with 14258-1-PBS in a different storage buffer formulation.

• Featured Product
• KD/KO Validated

MBD3 Polyclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA

Cat No. 14258-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, Indirect ELISA

Synonyms

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Product Information

14258-1-PBS targets MBD3 in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: MBD3 fusion protein Ag5531
• Full Name: methyl-CpG binding domain protein 3
• Calculated Molecular Weight: 33 kDa
• Observed Molecular Weight: 33 kDa
• GenBank Accession Number: BC043619
• Gene Symbol: MBD3
• Gene ID (NCBI): 53615
• RRID: AB_2139745
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: O95983
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

MBD3 does not bind DNA by itself. It recruits histone deacetylases and DNA methyltransferases. MBD3 acts as transcriptional repressor and plays a role in gene silencing.(PMID:9774669) .