MBD1 Polyclonal antibody

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  WB analysis using 29998-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 29998-1-AP (MBD1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  IF Staining of HeLa using 29998-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using MBD1 antibody (29998-1-AP) at dilution of 1:800 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-phalloidin (red).

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  IF Staining of MCF-7 using 29998-1-AP

  Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using MBD1 antibody (29998-1-AP) at dilution of 1:800 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002).

MBD1 Polyclonal Antibody for WB, IF/ICC, ELISA

Cat No. 29998-1-AP

Publications(1)

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, ELISA

Synonyms

MBD 1

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Tested Applications

• Positive WB detected in: HeLa cells, Jurkat cells
• Positive IF/ICC detected in: HeLa cells, MCF-7 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:8000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:400-1:1600

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below

Product Information

29998-1-AP targets MBD1 in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: MBD1 fusion protein Ag31424
• Full Name: methyl-CpG binding domain protein 1
• Calculated Molecular Weight: 67KD
• Observed Molecular Weight: 70-80 kDa
• GenBank Accession Number: NM_015846
• Gene Symbol: MBD1
• Gene ID (NCBI): 4152
• RRID: AB_2935497
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q9UIS9
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

MBD1, also known as RFT, PCM1, CXXC3, is a member of a family of nuclear proteins related by the presence of a methyl-CpG binding domain (MBD). MBD1 is a transcriptional repressor that binds CpG islands in promoters where the DNA is methylated at position 5 of cytosine within CpG dinucleotides. MBD1 acts as a transcriptional repressor and plays a role in gene silencing by recruiting AFT7IP, which in turn recruits factors such as the histone methyltransferase SETDB1. Five transcript variants of the MBD1 are generated by alternative splicing resulting in protein isoforms that contain one MBD domain, two to three cysteine-rich (CXXC) domains, and some differences in the COOH terminus. Isoform 1 and isoform 2 can repress transcription from unmethylated promoters (PMID: 12665582, 14610093, 16757475).

Publications

Species	Application	Title
human	WB	J Hematol Oncol; Novel potent molecular glue degraders against broad range of hematological cancer cell lines via multiple neosubstrates degradation; Authors - Pengyun Li