Myoglobin Polyclonal antibody

Myoglobin Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IHC, IF, IP, ELISA

Conjugate

Unconjugated

Publications

5

Cat no : 16048-1-AP

Synonyms

MB, MYO, myoglobin

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Validation Data Gallery

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  WB analysis using 16048-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 16048-1-AP (Myoglobin antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse skeletal muscle using 16048-1-AP

  IP result of anti-Myoglobin (IP:16048-1-AP, 4ug; Detection:16048-1-AP 1:1000) with mouse skeletal muscle tissue lysate 2000 ug.

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  IHC staining of human heart using 16048-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 16048-1-AP (Myoglobin antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human heart using 16048-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 16048-1-AP (Myoglobin antibody) at dilution of 1:100 (under 40x lens).

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Tested Applications

• Positive WB detected in: mouse heart tissue, mouse skeletal muscle, rat heart, rat skeletal muscle
• Positive IP detected in: mouse skeletal muscle tissue
• Positive IHC detected in: human heart tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:12000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 5 publications below
• IF: See 1 publications below

Product Information

16048-1-AP targets Myoglobin in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, pig
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Myoglobin fusion protein Ag9000
• Full Name: myoglobin
• Calculated Molecular Weight: 154 aa, 17 kDa
• Observed Molecular Weight: 18 kDa
• GenBank Accession Number: BC014547
• Gene Symbol: Myoglobin
• Gene ID (NCBI): 4151
• RRID: AB_1640050
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Myoglobin is a cytoplasmic hemoprotein that is expressed primarily in cardiomyocytes and oxidative skeletal muscle fibers, functioning on facilitating oxygen transport and modulating nitric oxide homeostasis within cardiac and skeletal myocytes. Recent studies indicated that myoglobin was also expressed in non-muscle tissues. This antibody well recognized endogenous myoglobin in muscle lysates.

Publications

Species	Application	Title
pig	WB	iScience; Dietary protein restriction regulates skeletal muscle fiber metabolic characteristics associated with the FGF21-ERK1/2 pathway; Authors - Shuo Li
mouse	WB	Physiol Rep; Effects of endurance training on the expression of host proteins involved in SARS-CoV-2 cell entry in C57BL/6J mouse.; Authors - Yuki Tamura
mouse	WB	Appl Physiol Nutr Metab; Exercise improves high-fat diet-induced metabolic disorder by promoting HDAC5 degradation through the ubiquitin-proteasome system in skeletal muscle; Authors - Song Huang
human	WB	J Invest Dermatol; N6-methyladenosine modification of lnc-CCKAR-5 regulates autophagy in human umbilical cord mesenchymal stem cells by destabilizing LMNA and inhibits diabetic wound healing; Authors - Jian Wang
human	WB,IF	Inflammation; RNA-seq Based Transcriptome Analysis Reveals Role of Myoglobin in Rheumatoid Arthritis; Authors - Haibin Wang