MAX Recombinant antibody

MAX Recombinant Antibody for WB, FC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

WB, FC, ELISA

Conjugate

Unconjugated

CloneNo.

230215E3

Cat no : 82958-1-RR

Synonyms

bHLHd4, bHLHd5, bHLHd6, bHLHd7, bHLHd8, MAX, MYC associated factor X, orf1, Protein max

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Validation Data Gallery

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  WB analysis using 82958-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 82958-1-RR (MAX antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  FC experiment of Raji using 82958-1-RR

  1x10^6 Raji cells were intracellularly stained with 0.4 ug MAX Recombinant antibody (82958-1-RR, Clone:230215E3) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.4 ug Isotype Control (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

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Tested Applications

• Positive WB detected in: PC-3 cells
• Positive FC detected in: Raji cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:8000
• Flow Cytometry (FC): FC : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

82958-1-RR targets MAX in WB, FC, ELISA applications and shows reactivity with Human samples.

• Tested Reactivity: Human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: MAX fusion protein Ag0680
• Full Name: MYC associated factor X
• Calculated Molecular Weight: 18 kDa
• Observed Molecular Weight: 22 kDa
• GenBank Accession Number: BC003525
• Gene Symbol: MAX
• Gene ID (NCBI): 4149
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Max, a member of MAX family, contains a basic helix-loop-helix (bHLH) domain, is a transcription regulator. MAX can form a sequence-specific DNA-binding protein complex with MYC or MAD which recognizes the core sequence 5'-CAC[GA]TG-3'. The MYC-MAX complex is a transcriptional activator, while the MAD-MAX complex is a repressor. MAX could dimerizated with another bHLH protein to form a heterodimer, such MYC or MAD. Thus bands recognized by this antibody much larger than predicted.