MATR3 Monoclonal antibody

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  WB analysis using 68280-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 68280-1-Ig (MATR3 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Alpha Tubulin Monoclonal antibody (HRP-66031) as loading control.

• 

  WB analysis using 68280-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 68280-1-Ig (MATR3 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IF Staining of HEK-293 using 68280-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed HEK-293 cells using MATR3 antibody (68280-1-Ig, Clone: 3B12C7 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1), CL594-phalloidin (red).

MATR3 Monoclonal Antibody for WB, IF/ICC, ELISA

Cat No. 68280-1-Ig

Clone No.3B12C7

Host / Isotype

Mouse / IgG2b

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, ELISA

Synonyms

Matrin-3, matrin 3, KIAA0723

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Tested Applications

• Positive WB detected in: LNCaP cells, rat brain tissue, A431 cells, HeLa cells, HEK-293 cells, Jurkat cells, K-562 cells, mouse brain tissue
• Positive IF/ICC detected in: HEK-293 cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

68280-1-Ig targets MATR3 in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Mouse / IgG2b
• Class: Monoclonal
• Type: Antibody
• Immunogen: MATR3 fusion protein Ag31502
• Full Name: matrin 3
• Calculated Molecular Weight: 847 aa, 95 kDa
• Observed Molecular Weight: 120 kDa
• GenBank Accession Number: BC015031
• Gene Symbol: MATR3
• Gene ID (NCBI): 9782
• RRID: AB_2935361
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P43243
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

MATR3, known as KIAA0723, is an 847 amino acid protein, which may play a role in transcription or may interact with other nuclear matrix proteins to form the internal fibrogranular network. MATR3 was originally identified as a nuclear matrix protein containing both DNA and RNA binding domains (PMID: 2033075) with a wide variety of functions ranging from DNA damage response to transcriptional and post-transcriptional regulation of cellular and viral RNAs(PMID: 21771346). the calculated molecular weight of MATR3 is 94 kDa, but we detect a 125 kDa protein as a result of PMID: 28977530.