MATN1 Recombinant antibody

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  WB analysis of mouse embryo using 84313-1-RR

  mouse embryo tissue were subjected to SDS PAGE followed by western blot with 84313-1-RR (MATN1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  Affinity and Kinetic Characterization of 84313-1-RR

  Biolayer interferometry (BLl) kinetic assays of 84313-1-RR against Human MATN1 were performed. The affinity constant is 1.14 nM.

MATN1 Recombinant Antibody for WB, ELISA

Cat No. 84313-1-RR

Clone No.241643D1

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, ELISA

Synonyms

Matrilin 1, CRTM, CMP, Cartilage matrix protein, 241643D1

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Tested Applications

• Positive WB detected in: mouse embryo tissue

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

84313-1-RR targets MATN1 in WB, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: MATN1 fusion protein Ag29386
• Full Name: matrilin 1, cartilage matrix protein
• Calculated Molecular Weight: 54 kDa
• Observed Molecular Weight: 54 kDa
• GenBank Accession Number: NM_002379
• Gene Symbol: MATN1
• Gene ID (NCBI): 4146
• RRID: AB_3671854
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P21941
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

MATN1 (matrilin 1), also known as CMP. It is expected to be located in extracellular space, the protein is mainly expressed in cartilage tissue and retina tissue. Also, the protein is a member of von Willebrand factor A domain containing protein family. This family of proteins are thought to be involved in the formation of filamentous networks in the extracellular matrices of various tissues. Mutations of this gene have been associated with variety of inherited chondrodysplasias. It is reproted that COMP and matrilin-1 elute together in the gel filtration of cartilage extracts and can be co-immunoprecipitated with the presence of calcium (PMID: 15075323). The calculated molecular weight of MATN1 is 54 kDa.