MATK Polyclonal antibody

MATK Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Publications

2

Cat no : 10082-2-AP

Synonyms

Tyrosine-protein kinase CTK, Megakaryocyte-associated tyrosine-protein kinase, HYL, Hematopoietic consensus tyrosine-lacking kinase, EC:2.7.10.2

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Validation Data Gallery

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  WB analysis of K-562 using 10082-2-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 10082-2-AP (MATK antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse thymus using 10082-2-AP

  mouse thymus tissue were subjected to SDS PAGE followed by western blot with 10082-2-AP (MATK antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  IHC staining of human breast cancer using 10082-2-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 10082-2-AP (MATK Antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human breast cancer using 10082-2-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 10082-2-AP (MATK Antibody) at dilution of 1:50 (under 40x lens).

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  IF Staining of HepG2 using 10082-2-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using MATK antibody (10082-2-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: K-562 cells, mouse thymus tissue
• Positive IHC detected in: human breast cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 2 publications below

Product Information

10082-2-AP targets MATK in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: MATK fusion protein Ag0128
• Full Name: megakaryocyte-associated tyrosine kinase
• Calculated Molecular Weight: 56 kDa
• Observed Molecular Weight: 56 kDa
• GenBank Accession Number: BC000114
• Gene Symbol: MATK
• Gene ID (NCBI): 4145
• RRID: AB_2141386
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

MATK(Megakaryocyte-associated tyrosine-protein kinase) is also named as CTK, HYL and belongs to the protein kinase superfamily. This protein can can inhibit the kinase activity of certain Src kinase family members by phosphorylating the C-terminal tyrosine and by a non-catalytic mechanism. It may play an inhibitory role in the control of T-cell proliferation. MATK has 3 isoforms produced by alternative splicing.

Publications

Species	Application	Title
	WB	Biomed Pharmacother; Anti-osteoporotic effects of alisol C 23-acetate via osteoclastogenesis inhibition.; Authors - Xiaokang Jia
human	WB	Biophys Rep; Detection of ribophagy in yeast and mammals; Authors - Miao Ye