MAT2B Polyclonal antibody

MAT2B Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (2)

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Cat no : 15952-1-AP

Synonyms

Putative dTDP-4-keto-6-deoxy-D-glucose 4-reductase, Nbla02999, MSTP045, MATIIbeta, MAT II beta



Tested Applications

Positive WB detected inHepG2 cells, HEK-293 cells, Jurkat cells, mouse brain tissue, mouse testis tissue
Positive IP detected inHepG2 cells
Positive IHC detected inhuman lung tissue, mouse lung tissue, human kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

15952-1-AP targets MAT2B in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat, pig, chicken
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen MAT2B fusion protein Ag8649
Full Name methionine adenosyltransferase II, beta
Calculated Molecular Weight 36 kDa, 38 kDa
Observed Molecular Weight 38 kDa
GenBank Accession NumberBC005218
Gene Symbol MAT2B
Gene ID (NCBI) 27430
RRIDAB_10637268
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for MAT2B antibody 15952-1-APDownload protocol
IHC protocol for MAT2B antibody 15952-1-APDownload protocol
IF protocol for MAT2B antibody 15952-1-APDownload protocol
IP protocol for MAT2B antibody 15952-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Mol Cell

mTORC1 stimulates cell growth through SAM synthesis and m6A mRNA-dependent control of protein synthesis.

Authors - Elodie Villa
human

Ann N Y Acad Sci

PMID: 29722030

Authors - Holly M Brown-Borg
ratWB

J Agric Food Chem

Mitotically stable modification of DNA methylation in IGF2/H19 imprinting control region is associated with activated hepatic IGF2 expression in offspring rats from betaine-supplemented dams.

Authors - Shu Yang
pigWB

J Agric Food Chem

Maternal Betaine Supplementation during Gestation Enhances Expression of mtDNA-Encoded Genes through D-Loop DNA Hypomethylation in the Skeletal Muscle of Newborn Piglets.

Authors - Yimin Jia
chickenWB

Sci Rep

In ovo injection of betaine alleviates corticosterone-induced fatty liver in chickens through epigenetic modifications.

Authors - Yun Hu
ratWB

J Nutr Biochem

Maternal betaine supplementation attenuates glucocorticoid-induced hepatic lipid accumulation through epigenetic modification in adult offspring rats.

Authors - Nannan Zhao