MAT2A Polyclonal antibody

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  WB analysis using 55309-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 55309-1-AP (MAT2A antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 55309-1-AP

  WB result of MAT2A antibody (55309-1-AP; 1:6000; incubated at room temperature for 1.5 hours) with sh-control and sh-MAT2A transfected HeLa cells.

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  IP experiment of HepG2 using 55309-1-AP

  IP result of anti-MAT2A (IP:55309-1-AP, 4ug; Detection:55309-1-AP 1:500) with HepG2 cells lysate 3200ug.

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  IHC staining of human stomach cancer using 55309-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 55309-1-AP (MAT2A antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human kidney using 55309-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 55309-1-AP (MAT2A antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon cancer using 55309-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 55309-1-AP (MAT2A antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human stomach cancer using 55309-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 55309-1-AP (MAT2A antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse liver using 55309-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 55309-1-AP (MAT2A antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of rat kidney using 55309-1-AP

  Immunohistochemical analysis of paraffin-embedded rat kidney tissue slide using 55309-1-AP (MAT2A antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HeLa using 55309-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using MAT2A antibody (55309-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• Featured Product
• KD/KO Validated

MAT2A Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Cat No. 55309-1-AP

Publications(21)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IHC, IF/ICC, IP, ELISA

Synonyms

AMS2, EC:2.5.1.6, MAT 2, MATA2, MATII

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Tested Applications

• Positive WB detected in: HEK-293 cells, HeLa cells, HepG2 cells, Jurkat cells, SGC-7901cells, NIH/3T3 cells
• Positive IP detected in: HepG2 cells
• Positive IHC detected in: human stomach cancer tissue, human colon cancer tissue, human kidney tissue, mouse liver tissue, rat kidney tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 5 publications below
• WB: See 18 publications below
• IHC: See 3 publications below
• IF: See 4 publications below

Product Information

55309-1-AP targets MAT2A in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat, pig
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  fusion protein

• Full Name: methionine adenosyltransferase II, alpha
• Calculated Molecular Weight: 44 kDa
• Observed Molecular Weight: 47 kDa, 49 kDa
• GenBank Accession Number: NM_005911
• Gene Symbol: MAT2A
• Gene ID (NCBI): 4144
• RRID: AB_2881303
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P31153
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

MAT2A, also named as AMS2, MATA2 and AdoMet synthase 2, belongs to the AdoMet synthase family. It catalyzes the formation of S-adenosylmethionine from methionine and ATP. This antibody is specific to MATA2.

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
human	WB	Mol Cell; mTORC1 stimulates cell growth through SAM synthesis and m6A mRNA-dependent control of protein synthesis.; Authors - Elodie Villa ; KD Validated
human	WB,IF	Oxid Med Cell Longev; Oxidative Stress Aggravates Apoptosis of Nucleus Pulposus Cells through m6A Modification of MAT2A Pre-mRNA by METTL16.; Authors - Peng-Bo Chen ; KD Validated
pig	WB	Antioxidants (Basel); M6A RNA Methylation Mediates NOD1/NF-kB Signaling Activation in the Liver of Piglets Challenged with Lipopolysaccharide; Authors - Menghui Xu
mouse	WB	FASEB J; Activating transcription factor 4 regulates angiogenesis under lipid overload via methionine adenosyltransferase 2A-mediated endothelial epigenetic alteration.; Authors - Li Chen ; KD Validated
mouse	WB,IF	FASEB J; Inhibition of MAT2A suppresses osteoclastogenesis and prevents ovariectomy-induced bone loss.; Authors - Honglei Kang ; KD Validated
rat	WB	Environ Toxicol Pharmacol; Ligustrazine modulates renal cysteine biosynthesis in rats exposed to cadmium.; Authors - Wenhua Kuang