Jurkat cells were subjected to SDS PAGE followed by western blot with 19507-1-AP (MAST3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
Jurkat cells were subjected to SDS PAGE followed by western blot with 19507-1-AP (MAST3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis using 19507-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 19507-1-AP (MAST3 antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours.
Various lysates were subjected to SDS PAGE followed by western blot with 19507-1-AP (MAST3 antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours.
WB analysis using 19507-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 19507-1-AP (MAST3 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.
Various lysates were subjected to SDS PAGE followed by western blot with 19507-1-AP (MAST3 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.
WB analysis of human brain using 19507-1-AP
human brain tissue were subjected to SDS PAGE followed by western blot with 19507-1-AP (MAST3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
human brain tissue were subjected to SDS PAGE followed by western blot with 19507-1-AP (MAST3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of human brain using 19507-1-AP
human brain tissue were subjected to SDS PAGE followed by western blot with 19507-1-AP (MAST3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
human brain tissue were subjected to SDS PAGE followed by western blot with 19507-1-AP (MAST3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of mouse lung using 19507-1-AP
mouse lung tissue were subjected to SDS PAGE followed by western blot with 19507-1-AP (MAST3 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
mouse lung tissue were subjected to SDS PAGE followed by western blot with 19507-1-AP (MAST3 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
IP experiment of Jurkat using 19507-1-AP
IP result of anti-MAST3 (IP:19507-1-AP, 4ug; Detection:19507-1-AP 1:500) with Jurkat cells lysate 4000ug.
Immunohistochemical analysis of paraffin-embedded human kidney using 19507-1-AP (MAST3 antibody) at dilution of 1:100 (under 40x lens).
IF Staining of HepG2 using 19507-1-AP
Immunofluorescent analysis of HepG2 cells, using MAST3 antibody 19507-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).
Immunofluorescent analysis of HepG2 cells, using MAST3 antibody 19507-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).
The Proteintech guarantee covers Proteintech antibodies in any species and any application, including those not listed on the datasheet. If the antibody doesn’t perform, you can receive a hassle-free refund or credit note.
Jurkat cells, human brain tissue, mouse lung tissue, mouse brain tissue, rat brain tissue
Positive IP detected in
Jurkat cells
Positive IHC detected in
human kidney tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected in
HepG2 cells
Recommended dilution
Application
Dilution
Western Blot (WB)
WB : 1:500-1:3000
Immunoprecipitation (IP)
IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)
IHC : 1:20-1:200
Immunofluorescence (IF)/ICC
IF/ICC : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.
Product Information
19507-1-AP targets MAST3 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage Conditions
Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.
Background Information
MAST3, also named as KIAA0561, belongs to the protein kinase superfamily and AGC Ser/Thr protein kinase family. MAST3 catalyze the reaction: ATP + a protein = ADP + a phosphoprotein.
Jurkat cells were subjected to SDS PAGE followed by western blot with 19507-1-AP (MAST3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis using 19507-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 19507-1-AP (MAST3 antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours.
WB analysis using 19507-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 19507-1-AP (MAST3 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.
WB analysis of human brain using 19507-1-AP
human brain tissue were subjected to SDS PAGE followed by western blot with 19507-1-AP (MAST3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of human brain using 19507-1-AP
human brain tissue were subjected to SDS PAGE followed by western blot with 19507-1-AP (MAST3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of mouse lung using 19507-1-AP
mouse lung tissue were subjected to SDS PAGE followed by western blot with 19507-1-AP (MAST3 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
IHC Figures
IHC staining of human kidney using 19507-1-AP
Immunohistochemical analysis of paraffin-embedded human kidney using 19507-1-AP (MAST3 antibody) at dilution of 1:100 (under 10x lens).
IHC staining of human kidney using 19507-1-AP
Immunohistochemical analysis of paraffin-embedded human kidney using 19507-1-AP (MAST3 antibody) at dilution of 1:100 (under 40x lens).
IP Figures
IP experiment of Jurkat using 19507-1-AP
IP result of anti-MAST3 (IP:19507-1-AP, 4ug; Detection:19507-1-AP 1:500) with Jurkat cells lysate 4000ug.
IF/ICC Figures
IF Staining of HepG2 using 19507-1-AP
Immunofluorescent analysis of HepG2 cells, using MAST3 antibody 19507-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).
The species listed in Tested Reactivity are in-house verified and applicable species. For unlisted species, please refer to the homology analysis of the immunogen sequence and related species. For rabbit polyclonal antibodies, homology >70% is recommended. For mouse monoclonal antibodies and rabbit recombinant antibodies, homology >90% is recommended. Generally, the higher the homology, the greater the applicability. However, there will be certain differences in protein expression in different species, tissues or cells. Therefore, the homology analysis results are for reference only and do not serve as a guarantee.
At Proteintech, we pride ourselves on our antibody quality, customer service and transparency. As such, we are comparing our antibodies with other vendors, enabling easy identification and comparisons of key data to help you choose the suitable antibody for your needs.
We have selected the top cited antibodies from these vendors for you to compare.
Proteintech
MAST3 Polyclonal antibody
Catalog Number
19507-1-AP
Citations
-
Dilutions
WB : 1:500-1:3000 IP : 0.5-4.0 ug for IP and 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate for WB IHC : 1:20-1:200 IF/ICC : 1:10-1:100
Applications
WB, IHC, IF/ICC, IP, ELISA
Reactivity
human, mouse, rat
Product Guarantee
Covers any species including not listed on datasheet
Covers any applications including not listed on datasheet
