MARVELD2 Polyclonal antibody

MARVELD2 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IF, IHC, ELISA

Conjugate

Unconjugated

Publications

5

Reviews (1)

Cat no : 13515-1-AP

Synonyms

DFNB49, MARVD2, MARVEL domain containing 2, MARVELD2, MRVLDC2, Tric, Tricellulin

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Validation Data Gallery

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  WB analysis using 13515-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 13515-1-AP (MARVELD2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis using 13515-1-AP

  Hela cell lysates were subjected to SDS PAGE followed by western blot with 13515-1-AP (MARVELD2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human stomach using 13515-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 13515-1-AP (MARVELD2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human stomach using 13515-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 13515-1-AP (MARVELD2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human small intestine using 13515-1-AP

  Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 13515-1-AP (MARVELD2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human small intestine using 13515-1-AP

  Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 13515-1-AP (MARVELD2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: HEK-293 cells, HeLa cells, mouse kidney tissue, rat kidney tissue
• Positive IHC detected in: human small intestine tissue, human stomach tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 2 publications below
• IHC: See 1 publications below
• IF: See 2 publications below

Product Information

13515-1-AP targets MARVELD2 in WB, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, pig
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: MARVELD2 fusion protein Ag4446
• Full Name: MARVEL domain containing 2
• Calculated Molecular Weight: 558 aa, 64 kDa
• Observed Molecular Weight: 64 kDa
• GenBank Accession Number: BC033689
• Gene Symbol: MARVELD2
• Gene ID (NCBI): 153562
• RRID: AB_2281702
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

MARVELD2, also named as Tricellulin or TRIC, is a 558 amino acid protein, which contains one MARVEL domain. MARVELD2 localizes the cell membrane and plays a role in the formation of the epithelial barriers. MARVELD2, is a first integral membrane protein that is concentrated at the vertically oriented tight junction strands of tricellular contacts. MARVELD2 may be a component to maintain the integrity for peripheral nervous system myelin function and morphology.

Publications

Species	Application	Title
mouse	WB	J Clin Invest; Tricellulin deficiency affects tight junction architecture and cochlear hair cells.; Authors - Nayak Gowri G
pig	WB,IHC	Microb Pathog; Alterations in the expression level of visfatin in the lungs of piglets infected with PRRSV and its effect on PRRSV replication.; Authors - Zhe-Wei Zhang
	IF	PLoS One; Lectin binding and gel secretion within Lorenzinian electroreceptors of Polyodon; Authors - David F. Russell
	IF	Tissue Cell; Papilla cells may guard the entrance to ampullary organs of Polyodon electroreceptors; Authors - David F Russell
human		Cell Rep; Alpha-tocopherylquinone differentially modulates claudins to enhance intestinal epithelial tight junction barrier via AhR and Nrf2 pathways; Authors - Ashwinkumar Subramenium Ganapathy

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Beth (Verified Customer) (10-05-2017)

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: RWPE1, PC-3, primary prostate epithelial cells

MARVELD2 Antibody Western Blot validation (1:1000 dilution) in RWPE1, PC-3, primary prostate epithelial cells (Cat no:13515-1-AP)