MARS Monoclonal antibody

MARS Monoclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

Human

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

3E3B4

Cat no : 67739-1-Ig

Synonyms

FLJ35667, MARS, Methionine tRNA ligase, methionyl tRNA synthetase, METRS, MTRNS

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Validation Data Gallery

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  WB analysis using 67739-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67739-1-Ig (MARS antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

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  WB analysis of A431 using 67739-1-Ig

  A431 cells were subjected to SDS PAGE followed by western blot with 67739-1-Ig (MARS antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

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  IF Staining of HepG2 using 67739-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using MARS antibody (67739-1-Ig, Clone: 3E3B4 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red).

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Tested Applications

• Positive WB detected in: HeLa cells, A431 cells, HepG2 cells, Jurkat cells, K-562 cells
• Positive IF/ICC detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

67739-1-Ig targets MARS in WB, IF/ICC, ELISA applications and shows reactivity with Human samples.

• Tested Reactivity: Human
• Host / Isotype: Mouse / IgG2a
• Class: Monoclonal
• Type: Antibody
• Immunogen: MARS fusion protein Ag6961
• Full Name: methionyl-tRNA synthetase
• Calculated Molecular Weight: 101 kDa
• Observed Molecular Weight: 97-101 kDa
• GenBank Accession Number: BC002384
• Gene Symbol: MARS
• Gene ID (NCBI): 4141
• RRID: AB_2918508
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.