MARCH8 Polyclonal antibody

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  WB analysis of mouse testis using 14119-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 14119-1-AP (MARCH8 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse testis using 14119-1-AP

  IP result of anti-MARCH8 (IP:14119-1-AP, 4ug; Detection:14119-1-AP 1:500) with mouse testis tissue lysate 1120 ug.

• Featured Product
• KD/KO Validated

MARCH8 Polyclonal Antibody for WB, IP, ELISA

Cat No. 14119-1-AP

Publications(27)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (1)

Applications

WB, IHC, IF, IP, CoIP, ELISA

Synonyms

MARCHF8, Membrane-associated RING finger protein 8, MARCH-VIII, EC:2.3.2.27, E3 ubiquitin-protein ligase MARCHF8

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Tested Applications

• Positive WB detected in: mouse testis tissue
• Positive IP detected in: mouse testis tissue

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 11 publications below
• WB: See 21 publications below
• IHC: See 2 publications below
• IF: See 2 publications below
• IP: See 3 publications below
• CoIP: See 1 publications below

Product Information

14119-1-AP targets MARCH8 in WB, IHC, IF, IP, CoIP, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, mouse, zebrafish
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: MARCH8 fusion protein Ag5268
• Full Name: membrane-associated ring finger (C3HC4) 8
• Calculated Molecular Weight: 33 kDa
• Observed Molecular Weight: 30-33 kDa
• GenBank Accession Number: BC066988
• Gene Symbol: MARCH8
• Gene ID (NCBI): 220972
• RRID: AB_2140168
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q5T0T0
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

MARCH8 (Membrane-associated RING finger protein 8) is also named as MIR, RNF178 and belongs to the RING zinc finger protein family. It functions as an E3 ubiquitin ligase for immune recognition-related molecules (e.g. major histocompatibility complex class I, B7-2, and ICAM-1). It plays a key role in controlling MHC class II surface expression by regulated ubiquitination of a lysine residue in the beta-chain (PMID:22761441).

Publications

Species	Application	Title
human	WB	Mol Cell; PMID: 28965816; Authors - Shouheng Jin ; KO Validated
human	WB	Nat Commun; MARCH8 inhibits influenza A virus infection by targeting viral M2 protein for ubiquitination-dependent degradation in lysosomes.; Authors - Xiaoman Liu
		Autophagy; BST2 suppresses porcine epidemic diarrhea virus replication by targeting and degrading virus nucleocapsid protein with selective autophagy.; Authors - Ning Kong ; KD Validated
human	WB	Autophagy; 2AB protein of Senecavirus A antagonizes selective autophagy and type I interferon production by degrading LC3 and MARCHF8.; Authors - Dage Sun ; KD Validated
human	WB	Proc Natl Acad Sci U S A; The E3 ubiquitin ligase MARCH8 negatively regulates IL-1β-induced NF-κB activation by targeting the IL1RAP coreceptor for ubiquitination and degradation.; Authors - Chen Rui R
human	WB	Cancers (Basel); MARCH8 Suppresses Tumor Metastasis and Mediates Degradation of STAT3 and CD44 in Breast Cancer Cells.; Authors - Wenjing Chen