EB1 Polyclonal antibody

• Featured Product
• KD/KO Validated

EB1 Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Conjugate

Unconjugated

Publications

5

Cat no : 17717-1-AP

Synonyms

MAPRE1, Microtubule-associated protein RP/EB family member 1, End-binding protein 1, End binding protein 1, APC-binding protein EB1

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Validation Data Gallery

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  WB analysis of HeLa using 17717-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 17717-1-AP (MAPRE1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 17717-1-AP

  WB result of EB1 antibody (17717-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-EB1 transfected HeLa cells.

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  WB analysis of A431 using 17717-1-AP

  A431 cells were subjected to SDS PAGE followed by western blot with 17717-1-AP (MAPRE1 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  WB analysis of Jurkat using 17717-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 17717-1-AP (MAPRE1 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa using 17717-1-AP

  IP result of anti-EB1 (IP:17717-1-AP, 4ug; Detection:17717-1-AP 1:1000) with HeLa cells lysate 1200ug.

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  IP experiment of Caco-2 using 17717-1-AP

  IP result of anti-EB1 (IP:17717-1-AP, 4ug; Detection:17717-1-AP 1:1000) with Caco-2 cells lysate 1920 ug.

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  IP experiment of mouse thymus using 17717-1-AP

  IP result of anti-EB1 (IP:17717-1-AP, 4ug; Detection:17717-1-AP 1:1000) with mouse thymus tissue lysate 2800ug.

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  IHC staining of human kidney using 17717-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 17717-1-AP (MAPRE1 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human kidney using 17717-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 17717-1-AP (MAPRE1 antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human colon using 17717-1-AP

  Immunohistochemical analysis of paraffin-embedded human normal colon slide using 17717-1-AP (EB1 antibody) at dilution of 1:300 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon using 17717-1-AP

  Immunohistochemical analysis of paraffin-embedded human normal colon slide using 17717-1-AP (EB1 antibody) at dilution of 1:300 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human placenta using 17717-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta using 17717-1-AP (MAPRE1 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human placenta using 17717-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta using 17717-1-AP (MAPRE1 antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human testis using 17717-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 17717-1-AP (MAPRE1 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human testis using 17717-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 17717-1-AP (MAPRE1 antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human skin using 17717-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin using 17717-1-AP (MAPRE1 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human skin using 17717-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin using 17717-1-AP (MAPRE1 antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human spleen using 17717-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 17717-1-AP (MAPRE1 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human spleen using 17717-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 17717-1-AP (MAPRE1 antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human lung using 17717-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung using 17717-1-AP (MAPRE1 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human lung using 17717-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung using 17717-1-AP (MAPRE1 antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human colon cancer using 17717-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 17717-1-AP (EB1 Antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human colon cancer using 17717-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 17717-1-AP (EB1 Antibody) at dilution of 1:50 (under 40x lens).

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  IF Staining of HeLa using 17717-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using EB1 antibody (17717-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

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  FC experiment of HeLa using 17717-1-AP

  1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human EB1 (17717-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Tested Applications

• Positive WB detected in: HeLa cells, Jurkat cells, A431 cells
• Positive IP detected in: HeLa cells, Caco-2 cells, mouse thymus tissue
• Positive IHC detected in: human kidney tissue, human placenta tissue, human testis tissue, human skin tissue, human spleen tissue, human lung tissue, human normal colon, human colon cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HeLa cells
• Positive FC (Intra) detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:3000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:100-1:400
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• IF: See 4 publications below

Product Information

17717-1-AP targets EB1 in WB, IHC, IF/ICC, FC (Intra), IP, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: EB1 fusion protein Ag12092
• Full Name: microtubule-associated protein, RP/EB family, member 1
• Calculated Molecular Weight: 268 aa, 30 kDa
• Observed Molecular Weight: 35 kDa
• GenBank Accession Number: BC106068
• Gene Symbol: EB1
• Gene ID (NCBI): 22919
• RRID: AB_2141624
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
human		Nat Commun; Microtubule asters anchored by FSD1 control axoneme assembly and ciliogenesis.; Authors - Hai-Qing Tu
human	IF	Elife; α-/γ-Taxilin are required for centriolar subdistal appendage assembly and microtubule organization.; Authors - Dandan Ma
mouse	IF	Biol Open; MACF1 promotes preosteoblast migration by mediating focal adhesion turnover through EB1; Authors - Peihong Su
mouse	IF	Biol Open; MACF1 promotes preosteoblast migration by mediating focal adhesion turnover through EB1.; Authors - Peihong Su
human	IF	Molecules; Pyrrolyldihydropyrazino[1,2-a]indoletrione Analogue Microtubule Inhibitor Induces Cell-Cycle Arrest and Apoptosis in Colorectal Cancer Cells; Authors - Dong-Lin Yang