MAPKAPK2 Recombinant antibody

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  WB analysis using 82929-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 82929-1-RR (MAPKAPK2 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  IF Staining of HeLa using 82929-1-RR

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using MAPKAPK2 antibody (82929-1-RR, Clone: 230197A9 ) at dilution of 1:150 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

MAPKAPK2 {ptg:Brand} Recombinant Antibody for WB, IF, ELISA

Cat No. 82929-1-RR

Clone No.230197A9

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

WB, IF, ELISA

Synonyms

MAPKAP kinase 2, MAPKAPK 2, MAPKAPK2, MK2

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Tested Applications

• Positive WB detected in: A549 cells, HeLa cells, HEK-293T cells
• Positive IF/ICC detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:3000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

82929-1-RR targets MAPKAPK2 in WB, IF, ELISA applications and shows reactivity with Human samples.

• Tested Reactivity: Human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: MAPKAPK2 fusion protein Ag34196
• Full Name: mitogen-activated protein kinase-activated protein kinase 2
• Calculated Molecular Weight: 400 aa, 46 kDa
• Observed Molecular Weight: 42-46 kDa
• GenBank Accession Number: BC036060
• Gene Symbol: MAPKAPK2
• Gene ID (NCBI): 9261
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P49137
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

MAPKAPK2(mitogen-activated protein kinase-activated protein kinase 2) is also named as MK2, MAPKAP-K2, MK-2 and belongs to the CAMK Ser/Thr protein kinase family. MAPKAPK2, one of several kinases directly phosphorylated and activated by p38 MAPK, plays a central role in the inflammatory response and is in the nucleus of unstimulated cells and moves rapidly to the cytoplasm after stimulation(PMID:12171911). It is also involved in many other cellular processes including stress responses, nuclear export, gene expression regulation and cell proliferation. Multiple residues of MAPKAPK2 are generally phosphorylated in vivo in response to stress, but only 4 residues(Thr25, Thr222, Ser272, and Thr334) are phosphorylated by p38 MAPK in vitro(PMID:22351694). It has 2 isoforms produced by alternative splicing and the range of the molecular weight is 42-60 kDa according to the references(PMID:10666409; 11328854;8995385).