SIN1 Polyclonal antibody

SIN1 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Publications

8

Cat no : 15463-1-AP

Synonyms

MAPKAP1, Target of rapamycin complex 2 subunit MAPKAP1, Stress-activated map kinase-interacting protein 1, SAPK interacting protein 1, Mitogen-activated protein kinase 2-associated protein 1

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Validation Data Gallery

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  WB analysis of mouse kidney using 15463-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 15463-1-AP (MAPKAP1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 15463-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 15463-1-AP (MAPKAP1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse kidney using 15463-1-AP

  IP result of anti-SIN1 (IP:15463-1-AP, 4ug; Detection:15463-1-AP 1:500) with mouse kidney tissue lysate 4000ug.

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  IHC staining of human kidney using 15463-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 15463-1-AP (MAPKAP1 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human kidney using 15463-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 15463-1-AP (MAPKAP1 antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of mouse kidney using 15463-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 15463-1-AP (SIN1 antibody) at dilution of 1:200 (under 40x lens).

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  IF Staining of HepG2 using 15463-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using SIN1 antibody (15463-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).

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Tested Applications

• Positive WB detected in: mouse kidney tissue, HeLa cells
• Positive IP detected in: mouse kidney tissue
• Positive IHC detected in: mouse kidney tissue, human kidney tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 6 publications below
• IHC: See 1 publications below
• IF: See 2 publications below

Product Information

15463-1-AP targets SIN1 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: SIN1 fusion protein Ag7753
• Full Name: mitogen-activated protein kinase associated protein 1
• Calculated Molecular Weight: 59 kDa
• Observed Molecular Weight: 65 kDa
• GenBank Accession Number: BC002326
• Gene Symbol: MAPKAP1
• Gene ID (NCBI): 79109
• RRID: AB_10598466
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
mouse	IF	Cell Rep; Mitochondrial dynamics define muscle fiber type by modulating cellular metabolic pathways; Authors - Tatsuki Yasuda
human	WB	EMBO Rep; Nup358 restricts ER-mitochondria connectivity by modulating mTORC2/Akt/GSK3β signalling; Authors - Misha Kalarikkal ; KD Validated
mouse	WB	Cell Rep; Hepatic DNAJB9 Drives Anabolic Biasing to Reduce Steatosis and Obesity.; Authors - Fangfang Sun
mouse	WB	bioRxiv; Host-derived oxidized phospholipids initiate effector-triggered immunity fostering lethality upon microbial encounter; Authors - Marco Di Gioia
mouse	IF	bioRxiv; Ibudilast Protects Retinal Bipolar Cells from Excitotoxic Retinal Damage and Activates the mTOR Pathway; Authors - Sumaya Hamadmad
human	WB,IHC	Cell Signal; Overexpression of microRNA-611 inhibits TGF-β-induced epithelial-mesenchymal transition and migration in lung cancer cells through MAPKAP1; Authors - Bin Ke