ERK5 Polyclonal antibody

ERK5 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Publications

1

Cat no : 11164-1-AP

Synonyms

ERK 5, MAPK7, MAPK 7, Extracellular signal-regulated kinase 5, ERK-5

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Validation Data Gallery

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  WB analysis of SKOV-3 using 11164-1-AP

  SKOV-3 cells were subjected to SDS PAGE followed by western blot with 11164-1-AP (ERK5 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse heart using 11164-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 11164-1-AP (ERK5 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 11164-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 11164-1-AP (ERK5 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse testis using 11164-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 11164-1-AP (ERK5 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IHC staining of human stomach using 11164-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 11164-1-AP (ERK5 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: SKOV-3 cells, HEK-293 cells, mouse heart tissue, mouse testis tissue
• Positive IHC detected in: human stomach tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below

Product Information

11164-1-AP targets ERK5 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: ERK5 fusion protein Ag1648
• Full Name: mitogen-activated protein kinase 7
• Calculated Molecular Weight: 89 kDa
• Observed Molecular Weight: 70 kDa
• GenBank Accession Number: BC009963
• Gene Symbol: MAPK7
• Gene ID (NCBI): 5598
• RRID: AB_2877749
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
human	WB	Cell; Systematic discovery of mutation-directed neo-protein-protein interactions in cancer.; Authors - Xiulei Mo