ERK5 Polyclonal antibody

ERK5 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, ELISA

Conjugate

Unconjugated

Publications

7

Cat no : 10036-2-AP

Synonyms

ERK 5, MAPK7, MAPK 7, Extracellular signal-regulated kinase 5, ERK-5

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Validation Data Gallery

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  WB analysis of mouse skeletal muscle using 10036-2-AP

  mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 10036-2-AP (ERK5 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of human heart using 10036-2-AP

  human heart tissue were subjected to SDS PAGE followed by western blot with 10036-2-AP (ERK5 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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Tested Applications

• Positive WB detected in: mouse skeletal muscle tissue, human heart tissue

Recommended dilution

• Western Blot (WB): WB : 1:200-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 7 publications below

Product Information

10036-2-AP targets ERK5 in WB, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: ERK5 fusion protein Ag0017
• Full Name: mitogen-activated protein kinase 7
• Calculated Molecular Weight: 89 kDa
• Observed Molecular Weight: 59 kDa
• GenBank Accession Number: BC007992
• Gene Symbol: MAPK7
• Gene ID (NCBI): 5598
• RRID: AB_2140439
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
mouse	WB	Redox Biol; p47phox deficiency impairs platelet function and protects mice against arterial and venous thrombosis.; Authors - Xiamin Wang
human	WB	Cancer Lett; The oncoprotein HBXIP enhances migration of breast cancer cells through increasing filopodia formation involving MEKK2/ERK1/2/Capn4 signaling.; Authors - Yinghui Li
human	WB	J Enzyme Inhib Med Chem; Design, synthesis and molecular mechanisms of novel dual inhibitors of heat shock protein 90/phosphoinositide 3-kinase alpha (Hsp90/PI3Kα) against cutaneous melanoma.; Authors - Feifei Qin
human	WB	Int J Hyperthermia; Decrease in MAP3Ks expression enhances the cell death caused by hyperthermia.; Authors - Atsushi Enomoto
mouse	WB	Gene; Intron retention is a stress response in sensor genes and is restored by Japanese herbal medicines: A basis for future clinical applications.; Authors - Trieu-Duc Vu
human	WB	Exp Ther Med; Proof-of-concept study investigating the role of S100P-RAGE in nasopharyngeal carcinoma.; Authors - Chengyu Wang