MAPK6 Polyclonal antibody

• 

  WB analysis of A431 using 12839-1-AP

  A431 cells were subjected to SDS PAGE followed by western blot with 12839-1-AP (MAPK6 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• Featured Product
• KD/KO Validated

MAPK6 Polyclonal Antibody for WB, ELISA

Cat No. 12839-1-AP

Publications(3)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF, IHC, ELISA

Synonyms

ERK 3, ERK3, HsT17250, MAP kinase 6, MAP kinase isoform p97, MAPK 6, MAPK6, p97 MAPK, p97MAPK, PRKM6

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Tested Applications

• Positive WB detected in: A431 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 2 publications below
• WB: See 3 publications below
• IHC: See 1 publications below
• IF: See 2 publications below

Product Information

12839-1-AP targets MAPK6 in WB, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: MAPK6 fusion protein Ag3577
• Full Name: mitogen-activated protein kinase 6
• Calculated Molecular Weight: 83 kDa
• Observed Molecular Weight: 100-105 kDa
• GenBank Accession Number: BC035492
• Gene Symbol: MAPK6
• Gene ID (NCBI): 5597
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q16659
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
rat	WB,IF	Sci Rep; MicroRNA-26a targets MAPK6 to inhibit smooth muscle cell proliferation and vein graft neointimal hyperplasia.; Authors - Juanjuan Tan ; KD Validated
human	WB,IHC,IF	J Exp Clin Cancer Res; LncMAPK6 drives MAPK6 expression and liver TIC self-renewal.; Authors - Guanqun Huang ; KO Validated
human	WB	Exp Ther Med; miR-98 inhibits cell proliferation and induces cell apoptosis by targeting MAPK6 in HUVECs.; Authors - Chuanxian Hu