MAPK4 Polyclonal antibody

MAPK4 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (1)

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Publications

4

Cat no : 26102-1-AP

Synonyms

ERK 4, ERK3, Erk4, MAP kinase 4, MAP kinase isoform p63, MAPK 4, MAPK4, p63 MAPK, p63MAPK, PRKM4

---

Validation Data Gallery

• 

  WB analysis of mouse brain using 26102-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 26102-1-AP (MAPK4 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human breast cancer using 26102-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 26102-1-AP (MAPK4 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 26102-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 26102-1-AP (MAPK4 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

---

Tested Applications

• Positive WB detected in: mouse brain tissue
• Positive IHC detected in: human breast cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:100-1:400

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 3 publications below
• IF: See 2 publications below

Product Information

The immunogen of 26102-1-AP is MAPK4 Fusion Protein expressed in E. coli.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, mouse, pig
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: MAPK4 fusion protein Ag22899
• Full Name: mitogen-activated protein kinase 4
• Calculated Molecular Weight: 587 aa, 66 kDa
• Observed Molecular Weight: 68 kDa
• GenBank Accession Number: BC050299
• Gene Symbol: MAPK4
• Gene ID (NCBI): 5596
• RRID: AB_2880378
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
mouse	IF	Cell Biosci; Identification of atypical mitogen-activated protein kinase MAPK4 as a novel regulator in acute lung injury.; Authors - Ling Mao
pig	WB	J Cell Physiol; MiR-127 attenuates adipogenesis by targeting MAPK4 and HOXC6 in porcine adipocytes.; Authors - Yun Gao
mouse	WB	Front Pharmacol; An Integrated Proteomics and Bioinformatics Approach Reveals the Anti-inflammatory Mechanism of Carnosic Acid.; Authors - Li-Chao Wang
human,mouse	WB,IF	Cancer Innov; MAPK4 facilitates angiogenesis by inhibiting the ERK pathway in non-small cell lung cancer; Authors - Jing Chen